猪丹毒新型灭活疫苗的研制及免疫效力评价

符雪艳; 张传健; 陈赛赛; 熊富强; 王继春

畜牧与兽医 ›› 2026, Vol. 58 ›› Issue (7) : 65 -70.

猪丹毒新型灭活疫苗的研制及免疫效力评价

符雪艳 ¹^,²^,³^,⁴^,^# ,
张传健 ¹^,²^,³^,⁵^,^# ,
陈赛赛 ¹^,²^,³ ,
熊富强 ⁴^,⁵^,^* ,
王继春 ¹^,²^,³^,^*

作者信息 +

Development and immunogenicity evaluation of a novel inactivated vaccine against Erysipelothrix rhusiopathiae

Xueyan FU ¹^,²^,³^,⁴ ,
Chuanjian ZHANG ¹^,²^,³^,⁵ ,
Saisai CHEN ¹^,²^,³ ,
Fuqiang XIONG ⁴^,⁵^,^* ,
Jichun WANG ¹^,²^,³^,^*

Author information +

文章历史 +

PDF (1199K)

摘要

旨在制备新型猪丹毒灭活疫苗并评价其对小鼠和仔猪的免疫效力。对分离的2株流行菌株AH-6和NJ-2进行生长动力学测定，2株菌在8 h达到生长稳定期。将2株菌培养至稳定期，甲醛灭活之后添加水溶性纳米佐剂IMS1313和免疫增强剂c制成新型猪丹毒灭活疫苗，疫苗的菌体抗原含量为5×10 ⁹ CFU/mL。疫苗检验合格后分别免疫小鼠和仔猪，评价其免疫效力。小鼠试验表明，AH-6和NJ-2灭活疫苗2次免疫的保护率均为100%，AH-6和NJ-2灭活疫苗一次免疫的保护率分别为80%和50%。基于小鼠试验结果，选择AH-6水溶性纳米佐剂灭活疫苗进行仔猪免疫效力评价，结果表明，该灭活疫苗可诱导高水平抗体，攻毒后对照组仔猪全部发病，而免疫组仔猪无临床症状和体温反应。血液菌体载量研究发现，攻毒对照组可检测出10 ²～10 ³ CFU/mL的含菌量，免疫组低于检测限。综上，猪丹毒AH-6水溶性纳米佐剂灭活疫苗免疫小鼠和断奶仔猪均可产生良好免疫保护效力，该结果可为猪丹毒的防控提供参考。

Abstract

This study aimed to prepare an inactivated vaccine against erysipelothrix rhusiopathiae and to evaluate its immunogenicity in mice and piglets. Growth kinetics analysis of two epidemic strains, AH-6 and NJ-2, revealed the fact that both the strains reached the stationary growth phase at 8 hours post-inoculation. The tested strains were cultured to the stationary growth phase, inactivated with formaldehyde, and formulated into an inactivated vaccine with aqueous phase nanoparticle adjuvant IMS1313 and immunopotentiator. The bacterial antigen content of this vaccine was quantified as 5×10 ⁹ CFU/mL. Following quality control testing, the vaccines were administered to mice and piglets to evaluate their immunogenicity. The mouse experiments demonstrated that both the inactivated AH-6 and NJ-2 vaccines achieved 100% protection rates with two-dose immunization. With a single dose, the AH-6 inactivated vaccine achieved an 80% protection rate, while the NJ-2 inactivated vaccine produced a 50% protection rate. Based on the mouse experiment results, the AH-6 inactivated vaccine was further selected for immunogenicity evaluation in piglets. The results showed that the AH-6 inactivated vaccine induced high antibody levels. After challenge, all piglets in the challenge control group developed clinical symptoms and their body temperature went higher, whereas the vaccinated group exhibited no clinical signs or temperature responses. Additionally, the blood bacterial load analysis revealed 10 ²-10 ³ CFU/mL in the challenge control group, while the vaccinated group remained below the detection limit. In conclusion, the AH-6 inactivated vaccine produced strong immune efficacy in both mice and weaned piglets. This study provided valuable insights into the prevention and control of swine erysipelas.

关键词

猪丹毒 / 灭活苗 / 免疫效力

Key words

swine erysipelas / inactivated vaccine / immunological efficacy

引用本文

引用格式 ▾

符雪艳,张传健,陈赛赛,熊富强,王继春. 猪丹毒新型灭活疫苗的研制及免疫效力评价[J]. 畜牧与兽医, 2026, 58(7): 65-70 DOI:

登录浏览全文

4963

注册一个新账户忘记密码

参考文献

原文顺序 | 出版日期 | 本文引用

[1]	OPRIESSNIG T, FORDE T, SHIMOJI Y. Erysipelothrix spp.: past, present, and future directions in vaccine research [J]. Front Vet Sci, 2020, 7: 174.

[2]	OPRIESSNIG T, COUTINHO T A . Erysipelas [M]// ZIMMERMAN J J, KARRIKER L A, RAMIREZ A, et al. Diseases of swine. Hoboken, New Jersey: John Wiley & Sons, Inc., 2019: 835-843.

[3]	HOFFMANN C, BILKEI G. Case study: chronic erysipelas of the sow-a subclinical manifestation of reproductive problems [J]. Reprod Domest Anim, 2002, 37(2): 119-120.

[4]	BENDER J S, IRWIN C K, SHEN H G, et al. Erysipelothrix spp. genotypes, serotypes, and surface protective antigen types associated with abattoir condemnations [J]. J Vet Diagn Invest, 2011, 23(1): 139-142.

[5]	HU X, ZHOU Q, LUO Y. Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China [J]. Environ Pollut, 2010, 158(9): 2992-2998.

[6]	DEC M, NOWAK T, WEBSTER J, et al. Serotypes, antimicrobial susceptibility, and potential mechanisms of resistance gene transfer in Erysipelothrix rhusiopathiae strains from waterfowl in Poland [J]. Int J Mol Sci, 2024, 25(22): 12192.

[7]	IMADA Y, TAKASE A, KIKUMA R, et al. Serotyping of 800 strains of Erysipelothrix isolated from pigs affected with erysipelas and discrimination of attenuated live vaccine strain by genotyping [J]. J Clin Microbiol, 2004, 42(5): 2121-2126.

[8]	GHATTAS M, DWIVEDI G, LAVERTU M, et al. Vaccine technologies and platforms for infectious diseases: current progress, challenges, and opportunities [J]. Vaccines, 2021, 9(12): 1490.

[9]	GUAN L J, PEI S X, SONG J J, et al. Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae [J]. Front Vet Sci, 2022, 9: 922867.

[10]	STEPHENSON E H, BERMAN D T. Isolation of Erysipelothrix rhusiopathiae from tonsils of apparently normal swine by two methods [J]. Am J Vet Res, 1978, 39(1): 187-188.