驴食草酚对过氧化氢引起人脐静脉内皮细胞损伤的保护作用

摘要

目的 探讨驴食草酚（vestitol）对过氧化氢（H₂O₂）引起人脐静脉内皮细胞（HUVEC）损伤的保护作用。方法 体外培养HUVEC,通过设置700~900 μmol/L H₂O₂系列浓度筛选H₂O₂损伤HUVEC浓度。设置1.25~20 mg/L驴食草酚系列浓度筛选驴食草酚对HUVEC细胞毒作用。H₂O₂作用于HUVEC,评价驴食草酚对H₂O₂引起HUVEC损伤的保护作用。银杏叶提取物（GBE）为阳性对照药。倒置显微镜观察细胞形态,CCK-8法检测细胞存活率,丙酮酸还原法检测乳酸脱氢酶（LDH）释放量。结果 随着H₂O₂浓度的增加,HUVEC的受损程度逐渐增加,细胞形态发生异常改变，细胞存活率逐渐降低，LDH释放量逐渐增加，综合确定H₂O₂损伤HUVEC浓度为750 μmol/L。1.25～10 mg/L驴食草酚对HUVEC无细胞毒性，20 mg/L驴食草酚对HUVEC开始产生毒作用，最高无毒浓度为10 mg/L。驴食草酚可改善H₂O₂所致HUVEC细胞形态异常改变，提高HUVEC细胞存活率，减少LDH释放量。结论 驴食草酚可有效减轻H₂O₂引起的HUVEC损伤。

关键词

驴食草酚 / 人脐静脉内皮细胞 / 过氧化氢

[1] 邱雅慧. 血管内皮细胞的功能以及损伤修复与动脉粥样硬化[J]. 中国组织工程研究与临床康复,2007,11(10):1927-1929,1933.
[2] 罗磊,姬青华,马丽苹,等. 绿豆皮黄酮对H₂O₂诱导人脐静脉血管内皮细胞损伤的保护作用[J]. 中国食品学报,2020,20(2):35-41.
[3] Shen YF, Wu X, Cao G, et al.Effect of 5-HMF on protection of hydrogen peroxide-induced injury in human umbilical vein endothelial cells[J]. J Chin Pharm Sci, 2015, 24(3): 164-168.
[4] Wang Z, Wang Y, Chen Y, et al.The IL-24 gene protects human umbilical vein endothelial cells against H2O2-induced injury and may be useful as a treatment for cardiovascular disease[J]. Int J Mol Med, 2016, 37(3): 581-592.
[5] Franchin M, Cólon DF, Castanheira FV, et al.Vestitol isolated from brazilian red propolis inhibits neutrophils migration in the inflammatory process: elucidation of the mechanism of action[J]. J Nat Prod, 2016, 79(4): 954-960.
[6] 马沛广,李秋逸,刘佳静,等. 驴食草酚调控ERK1/2和p38 MAPK信号通路防治溃疡性结肠炎的研究[J]. 现代中西医结合杂志,2022,31(20):2803-2807,2889.
[7] 姜爱莉,孙利芹. 降香抗氧化成分的提取及活性研究[J]. 精细化工,2004,21(7):525-528.
[8] Bueno-Silva B, Alencar SM, Koo H, et al.Anti-inflammatory and antimicrobial evaluation of neovestitol and vestitol isolated from Brazilian red propolis[J]. Agric Food Chem, 2013, 61(19): 4546-4550.
[9] 王如科,孙源源,韩建一,等. 银杏叶提取物对过氧化氢刺激下的血管内皮细胞的影响[J]. 现代生物医学进展,2014,14(26):5038-5041,5048.
[10] Kim SJ, Mesquita FCP, Hochman-Mendez C.New biomarkers for crdiovascular disease[J]. Tex Heart Inst J, 2023, 50(5): e238178.
[11] Kim H, Yun J, Kwon SM.Therapeutic strategies for oxidative stress-related cardiovascular diseases: removal of excess reactive oxygen species in adult stem cells[J]. Oxid Med Cell Longev, 2016, 2016(5): 2483163.
[12] Kelly FJ, Fussell JC.Role of oxidative stress in cardiovas cular disease outcomes following exposure to ambient air pollution[J]. Free Radical Biol Med, 2017, 110: 345-367.
[13] 王燕,补蔚萍,谢宏,等. 大豆异黄酮对过氧化氢及脂多糖诱导的血管内皮细胞损伤保护机制的实验研究[J]. 中华心血管病杂志,2014,42(2):150-155.
[14] Yokoyama M.Oxidant stress and atherosclerosis[J]. Current Opin Pharm acol, 2004, 4: 110-119.
[15] Taniyama Y, Griendling KK.Reactive oxygen species in the vasculature molecular and cellular mechanisms[J]. Hypertension, 2003, 42: 1075-1081.
[16] Zhong Z, Chen X, Tan W, et al.Germacrone inhibits the proliferation of breast cancer cell lines by inducing cell cycle arrest and promoting apoptosis[J]. Eur J Pharmacol, 2011, 667(1-3): 50-55.

基金资助

河北省中药研究与开发重点实验室开放性课题(ZSKF202305)