SEMA3F、ATF4在喉癌组织中的表达及对术后复发转移的预测

Expression of SEMA3F and ATF4 in Laryngeal Carcinoma and Prediction of Postoperative Recurrence and Metastasis

摘要

目的 探究神经轴突导向因子（SEMA3F）、转录激活因子4（ATF4）在喉癌组织中表达及对患者术后复发转移的预测价值。方法 选取2016年8月~2022年8月于莆田学院附属医院进行喉癌手术治疗的患者60例,将经病理学证实出现术后复发转移的37例患者分为复发转移组,其余未出现术后复发转移的23例患者分为未复发转移组。免疫组化法测定SEMA3F、ATF4蛋白阳性率;RT-PCR法检测SEMA3F、ATF4表达水平。分析SEMA3F、ATF4对喉癌患者术后复发转移的预测价值。结果 与非复发转移组对比,复发转移组患者喉癌组织中的SEMA3F蛋白阳性表达降低、ATF4蛋白阳性表达升高;SEMA3F mRNA相对表达量降低,ATF4 mRNA相对表达量升高（P<0.05）。与未复发转移组相比,复发转移组癌胚抗原（CEA）、糖类抗原199（CA199）、糖类抗原125（CA125）表达水平降低（P<0.05）。Logistic回归分析显示,CEA、CA199、CA125、SEMA3F、ATF4是喉癌患者术后复发转移的独立危险因素。ROC曲线显示,联合检测预测喉癌术后复发转移的诊断效能显著高于SEMA3F、ATF4单一检测。结论 SEMA3F、ATF4是喉癌患者术后复发转移的风险预测因子,对喉癌术后复发转移具有较高的预测价值。

Abstract

Objective Exploration (SEMA3F), transcriptional activator 4 (ATF 4) expression in laryngeal cancer tissue and predictive value of postoperative recurrence and metastasis. Methods A total of 60 patients who were treated for laryngeal cancer in the Affiliated Hospital of Putian University from August 2016 to August 2022, 37 patients with pathologically confirmed postoperative recurrence and metastasis were divided into recurrence and metastasis group, and the other 23 patients without postoperative recurrence and metastasis were divided into no recurrence and metastasis group. The positive rates of SEMA3F and ATF 4 were determined by immunohistochemistry and SEMA3F and ATF 4 by RT-PCR. The predictive value of SEMA3F and ATF 4 for postoperative recurrence and metastasis in patients with laryngeal cancer was analyzed. Results Compared with the non-recurrent metastasis group, SEMA3F expression and ATF 4 expression decreased in laryngeal carcinoma tissue; SEMA3F mRNA expression decreased and ATF4 mRNA expression increased (P<0.05). Compared with the unmetastatic group, the expression levels of carcinoembryonic antigen (CEA), sugar antigen 199 (CA199), and sugar antigen 125 (CA125) were decreased in the metastatic group (P<0.05). Logistic Regression analysis showed that CEA, CA199, CA125, SEMA3F, and ATF 4 were independent risk factors for postoperative recurrence and metastasis in patients with laryngeal cancer. The ROC curve showed that the diagnostic efficacy of the combination test to predict recurrence and metastasis after laryngeal cancer was significantly higher than the single test of SEMA3F and ATF 4. Conclusion SEMA3F, ATF 4 is a risk predictor of postoperative recurrence and metastasis in laryngeal cancer patients, and it has a higher application value in the diagnosis of recurrence and metastasis after laryngeal cancer surgery.

关键词

神经轴突导向因子 / 转录激活因子4 / 喉癌 / 复发

Key words

Semaphorin 3F / Transcriptional activator 4 / Laryngeal cancer / Recrudescence

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