结直肠癌中BAIAP2L2的表达研究

Expression of BAIAP2L2 in Colorectal Cancer

摘要

目的 观察结直肠癌（CRC）组织中BAIAP2L2蛋白的表达情况,并探讨其表达情况与临床病理资料的关系。方法 选取CRC患者31例、结直肠息肉（CP）患者33例,手术中留取切除的肿瘤组织分别记为CRC组、CP组,留取CRC患者的癌旁组织（距癌组织>5cm的正常黏膜组织）记为Control组。采用免疫组织化学染色法检测各组组织中BAIAP2L2蛋白,采用半定量评分法观察蛋白表达情况,包括阳性表达率、阳性强度,并分析BAIAP2L2蛋白表达与CRC患者临床相关参数的关系。结果 BAIAP2L2蛋白在癌旁组织以及息肉组织中呈现不染色或染色颜色较浅、阳性面积小的染色结果,而在CRC组织中染色较深,多数呈黄色或棕黄色颗粒沉积,提示其在CRC组织表达较高。CRC组、CP组、Control组BAIAP2L2蛋白阳性表达率分别为96.8%（30/31）、24.2%（8/33）、3.2%（1/31）,3组间阳性表达率存在显著差异（P<0.001）。3组之间两两比较,BAIAP2L2蛋白阳性强度在CRC组与CP组、CRC组与Control组呈现差异表达（均P<0.001）,但CP组与Control组差异无统计学意义（P>0.05）。结论 CRC组织中BAIAP2L2蛋白呈现高表达,且阳性强度与CP组织及癌旁组织存在显著差异。

Abstract

Objective To observe the expression changes of BAIAP2L2 protein in colorectal cancer(CRC)tissues, and the relationship between its expression and clinicopathological data was discussed. Methods Thirty-one patients with CRC and 33 patients with colorectal polyps were selected. The tumor tissues removed during the operation were recorded as CRC group and CP group, and the normal adjacent mucosal tissues (the normal tissue >5cm away from the cancer tissue) of colorectal cancer patients were recorded as the Control group. Immunohistochemical staining was used to detect BAIAP2L2 protein in the tissues of each group, and semi-quantitative scoring was used to observe the protein expression, including the positive expression rate, area of positive expression and the degree of positivity, and to analyze the relationship between the expression of BAIAP2L2 protein and the clinically relevant parameters of CRC patients. Results BAIAP2L2 protein showed no staining or lighter staining color and small positive area in paracarcinoma tissues as well as polyp tissues, whereas in colorectal cancer tissues the staining was darker, and most of them showed yellow or brownish-yellow granule deposition, suggesting that its expression was higher in CRC tissues. The positive expression rates of BAIAP2L2 proteins in the CRC group, CP group and Control group were 96.8% (30/31), 24.2% (8/33) and 3.2% (1/31), respectively, and there was a significant difference in the positive expression rates among the three groups (P<0.001). The positive intensity of BAIAP2L2 protein was differently expressed between CRC group and CP group, CRC group and Control group (all P<0.001), but there was no statistical difference between CP group and Control group (P>0.05). Conclusion The expression of BAIAP2L2 protein in CRC tissues is highly expressed, and the positive intensity is significantly different from that of colorectal polyp tissues and adjacent tissues.

关键词

结直肠癌 / BAIAP2L2 / 结直肠息肉 / 癌旁组织 / 免疫组织化学染色法

Key words

Colorectal cancer / BAIAP2L2 / Colorectal polyps / Adjacent mucosal tissues / Immunohistochemical staining

[1] Sung H, Ferlay J, Siegel RL, et al.Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[2] Li N, Lu B, Luo C, et al.Incidence, mortality, survival, risk factor and screening of colorectal cancer: A comparison among China, Europe, and northern America[J]. Cancer Lett, 2021, 522: 255-268.
[3] Dekker E, Tanis PJ, Vleugels JLA, et al.Colorectal cancer[J]. Lancet, 2019, 394(10207): 1467-1480.
[4] Pan J, Cen L, Xu L, et al.Prevalence and risk factors for colorectal polyps in a Chinese population: a retrospective study[J]. Sci Rep, 2020, 10(1): 6974.
[5] Zhao H, Ming T, Tang S, et al.Wnt signaling in colorectal cancer: pathogenic role and therapeutic target[J]. Mol Cancer, 2022, 21(1): 144.
[6] Pykäläinen A, Boczkowska M, Zhao H, et al.Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures[J]. Nat Struct Mol Biol, 2011, 18(8): 902-907.
[7] Liu J, Shangguan Y, Sun J, et al.BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways[J]. Biomed Pharmacother, 2020, 129: 110414.
[8] Tang Z, Li C, Kang B, et al.GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses[J]. Nucleic Acids Res, 2017, 45(W1): W98-W102.
[9] Cancer Genome Atlas Network. Comprehensive molecular characterization of human colon and rectal cancer[J]. Nature, 2012, 487(7407): 330-337.
[10] Li Q, Lai Q, He C, et al.RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer[J]. J Exp Clin Cancer Res, 2019, 38(1):334.
[11] Long X, Hu Y, Duan S, et al.MRGBP promotes colorectal cancer metastasis via DKK1/Wnt/β-catenin and NF-kB/p65 pathways mediated EMT[J]. Exp Cell Res, 2022, 421(1): 113375.
[12] Liu L, Zhang Y, Wong CC, et al.RNF6 Promotes Colorectal Cancer by Activating the Wnt/β-Catenin Pathway via Ubiquitination of TLE3[J]. Cancer Res, 2018, 78(8): 1958-1971.
[13] Xu L, Du H, Zhang Q, et al.BAI1associated protein 2like 2 is a potential biomarker in lung cancer[J]. Oncol Rep, 2019, 41(2): 1304-1312.
[14] Liu S, Wang W, Zhao Y, et al.Identification of Potential Key Genes for Pathogenesis and Prognosis in Prostate Cancer by Integrated Analysis of Gene Expression Profiles and the Cancer Genome Atlas[J]. Front Oncol, 2020, 10: 809.
[15] Song Y, Zhuang G, Li J, et al.BAIAP2L2 facilitates the malignancy of prostate cancer (PCa) via VEGF and apoptosis signaling pathways[J]. Genes Genomics, 2021, 43(4): 421-432.
[16] Liu R, Hu Y, Liu T, et al.Profiles of immune cell infiltration and immune-related genes in the tumor microenvironment of osteosarcoma cancer[J]. BMC Cancer, 2021, 21(1): 1345.
[17] Chao A, Tsai CL, Jung SM, et al.BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer[J]. PLoS One, 2015, 10(7): e0133081.
[18] Wang YP, Huang LY, Sun WM, et al.Insulin receptor tyrosine kinase substrate activates EGFR/ERK signalling pathway and promotes cell proliferation of hepatocellular carcinoma[J]. Cancer Lett, 2013, 337(1): 96-106.
[19] Cappell MS. The pathophysiology, clinical presentation,diagnosis of colon cancer and adenomatous polyps[J]. Med Clin North Am, 2005, 89(1): 1-42, vii.
[20] Lee JK, Jensen CD, Levin TR, et al.Long-term Risk of Colorectal Cancer and Related Death After Adenoma Removal in a Large, Community-based Population[J]. Gastroenterology, 2020, 158(4): 884-894.
[21] 郑晓芳,王锦玉,李子鑫,等. 结直肠侧向发育型肿瘤组织中RNF6蛋白的表达变化及其意义[J].山东医药,2023,63(9):36-40.