EID2B在结肠癌中的表达及与细胞迁移的相关性分析

Correlation between EID2B expression and cell migration in colon cancer

摘要

目的 探究EID2B在结肠癌（COAD）免疫微环境中对肿瘤细胞迁移的影响。方法 通过TCGA数据库分析EID2B在COAD中的表达水平,及其与临床分期及生存曲线的关系;HPA数据库分析EID2B的蛋白表达水平;TIMER数据库分析EID2B与免疫细胞浸润的相关性;TCGA数据库进行EID2B生物学过程富集分析;利用体外实验设计siRNA转染THP-1诱导的巨噬细胞以敲减EID2B表达,RT-qPCR检测EID2B敲减效率。划痕实验、Transwell实验评估对HCT116细胞迁移能力的影响。结果 EID2B在COAD中呈低表达（P<0.05）,与淋巴结转移和M2巨噬细胞的浸润水平呈负相关（P<0.05）;敲减EID2B的巨噬细胞培养上清可促进HCT116的迁移（P<0.05）。结论 EID2B低表达与COAD淋巴结转移相关,敲减EID2B可促进肿瘤迁移。

Abstract

Objective To investigate whether EID2B affects tumor cell migration within the colon adenocarcinoma (COAD) immune microenvironment, and to provide a reference for subsequent research in this field. Methods The expression levels of EID2B in COAD were analyzed using the TCGA database, along with its relationship to clinical staging and survival curves; the HPA database was interrogated for analyze the protein expression levels of EID2B; the TIMER database was utilized to analyze the correlation between EID2B and immune cell infiltration; the TCGA database was applied to enrichment analysis of EID2B biological processes; in vitro experiments involved the design of siRNA transfection in THP-1 induced macrophages to knock down EID2B expression, with RT-qPCR detecting the efficiency of EID2B knockdown. Scratch assays and Transwell assays were conducted to evaluate the impact on the migration ability of HCT116 cells. Results EID2B is underexpressed in COAD (P<0.05), correlated with lymph node metastasis (P<0.05), and significantly correlated with macrophage infiltration (P<0.05). The culture supernatant from EID2B knockdown macrophages promoted the migration of HCT116 cells (P<0.05). Conclusion EID2B underexpression is associated with lymph node metastasis in COAD, and knocking down EID2B promotes tumor migration.

关键词

EID2B / 结肠癌 / 预后 / 免疫细胞浸润 / 巨噬细胞

Key words

EID2B / colon adenocarcinoma / prognosis / immune cell infiltration / macrophage

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基金资助

承德医学院重点学科资助-病理学与病理生理学; 河北省科学技术厅重点研发项目：卫生健康创新专项（21377791D）; 河北省高等学校科学技术研究项目（ZD2022124）; 河北省硕士在读研究生创新能力培养资助项目（CXZZSS2024117）