胃癌组织中MLF1IP、NKD1、GOLPH3表达及与免疫细胞浸润水平和药物敏感度的关系

Expression of MLF1IP, NKD1 and GOLPH3 in gastric cancer tissues and relationship with immune cell infiltration level and drug sensitivity

摘要

目的 检测胃癌组织中骨髓增生异常白血病因子1相互作用蛋白（MLF1IP）、裸角质膜同源蛋白1（NKD1）、高尔基体磷蛋白3（GOLPH3）表达情况,并判断与其免疫细胞浸润水平、药物敏感度之间的关系。方法 选取2022年1月—2024年1月于商丘市第一人民医院接受治疗的51例胃癌患者的癌及癌旁组织,利用免疫组化法检测GOLPH3、MLF1IP、NKD1表达,分析其表达情况与免疫细胞浸润水平、不同药物敏感度之间的关系。结果 与癌旁组织相比,胃癌组织GOLPH3表达、巨噬细胞水平、NKD1表达、MLF1IP表达较高,CD8⁺T细胞、B细胞、CD4⁺T细胞、中性粒细胞、树突状细胞水平较低,差异具有统计学意义（P<0.05）;胃癌组织阿霉素（ADM）、顺铂（DDP）耐药性高于氟尿嘧啶（5-FU）、丝裂霉素（MMC）,差异有统计学意义（P<0.05）;NKD1、MLF1IP、GOLPH3与CD8⁺T细胞、中性粒细胞、B细胞、CD4⁺T细胞、树突状细胞水平呈负相关,与巨噬细胞水平呈正相关;与NKD1、MLF1IP阴性表达相比,NKD1、MLF1IP阳性表达MMC、DDP、5-FU、ADM耐药性高、敏感度低;与GOLPH3阴性表达相比,GOLPH3阳性表达ADM、5-FU、DDP、MMC敏感度高、耐药性低,差异有统计学意义（P<0.05）。结论 MLF1IP、NKD1、GOLPH3在胃癌组织呈高表达,与化疗药物敏感度有密切联系,且可抑制免疫细胞浸润。

Abstract

Objective To detect the expression of myelodysplastic leukemia factor 1-interacting protein (MLF1IP), naked cuticle homolog protein 1 (NKD1), and Golgi phosphoprotein 3 (GOLPH3) in gastric cancer tissues, and to determine the relationship between them and the level of immune cell infiltration and drug sensitivity. Methods The research subjects were the cancer and adjacent tissues of 51 patients with gastric cancer who were treated at the First People's Hospital of Shangqiu City from January 2022 to January 2024. The expressions of GOLPH3, MLF1IP and NKD1 were detected by immunohistochemistry. Analyze the relationship between the expression of MLF1IP, NKD1 and GOLPH3 and the infiltration level of immune cells as well as the sensitivity to different drugs. Results Compared with adjacent tissues, the expression of GOLPH3, the level of macrophages, the expression of NKD1 and the expression of MLF1IP in gastric cancer tissues were higher, while the levels of CD8⁺T cells, B cells, CD4⁺T cells, neutrophils and dendritic cells were lower. The differences were statistically significant (P<0.05). The drug resistance of doxorubicin (ADM) and cisplatin (DDP) in gastric cancer tissues was higher than that of fluorouracil (5-FU) and mitomycin (MMC), and the difference was statistically significant (P<0.05). NKD1, MLF1IP and GOLPH3 were negatively correlated with CD8⁺T cells, neutrophils, B cells, CD4+T cells and dendritic cells, and positively correlated with macrophages. Compared with the negative expressions of NKD1 and MLF1IP, the positive expressions of NKD1 and MLF1IP have higher drug resistance and lower sensitivity for MMC, DDP, 5-FU and ADM. Compared with the negative expression of GOLPH3, the positive expression of GOLPH3 had higher sensitivity and lower drug resistance in ADM, 5-FU, DDP and MMC, and the difference was statistically significant (P<0.05). Conclusion MLF1IP, NKD1 and GOLPH3 are highly expressed in gastric cancer tissues, closely related to the sensitivity of chemotherapy drugs, and can inhibit the infiltration of immune cells.

关键词

胃癌组织 / MLF1IP / GOLPH3 / NKD1 / 免疫细胞浸润

Key words

gastric cancer tissue / MLF1IP / GOLPH3 / NKD1 / immune cell infiltration / drug sensitivity

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