半夏提取物对小鼠的抗炎镇痛活性及机制研究

Study on the anti-inflammatory and analgesic activities and mechanisms of Pinellia ternata extract in mice

摘要

目的 研究半夏提取物的抗炎镇痛活性及其抗炎活性的可能机制。方法 将小鼠随机分为5组：对照组（0.02 mL·g^-1生理盐水）,半夏提取物高、中、低剂量组（100、50、25 mg·kg^-1）和阿司匹林组（100 mg·kg^-1）,每组10只。各组小鼠每日灌胃给药1次,连续3 d,末次给药后1 h进行后续实验。通过二甲苯致小鼠耳廓肿胀实验,结合ELISA法测定小鼠血清肿瘤坏死因子-α（TNF-α）、前列腺素E2（PGE2）和一氧化氮（NO）含量,观察半夏提取物的抗炎活性及可能机制。通过小鼠醋酸扭体实验和热板实验观察半夏提取物的镇痛作用。结果 半夏提取物表现出一定的抗炎镇痛活性：与对照组比较,半夏提取物中、高剂量组小鼠耳廓肿胀度,小鼠扭体次数,血清TNF-α、PGE2和NO含量均明显降低,差异具有统计学意义（P<0.05）;半夏提取物中、高剂量组小鼠痛阈值明显延长,差异具有统计学意义（P<0.05）。随着半夏提取物剂量的增加,小鼠的耳廓肿胀抑制率、镇痛率均逐渐升高。结论 半夏提取物具有一定的抗炎镇痛作用,其抗炎机制可能与下调血清TNF-α、PGE2和NO的含量有关。

Abstract

Objective To study the anti-inflammatory and analgesic activities of Pinellia ternata extract and its potential anti-inflammatory mechanisms. Methods Mice were randomly divided into 5 groups with 10 mice in each group: control group (saline, 0.02 mL·g^-1), high-, medium-, and low-dose Pinellia ternata extract group (100, 50, 25 mg·kg^-1 respectively), and aspirin group (100 mg·kg^-1). The mice in each group were administered with corresponding drugs by oral gavage once daily for 3 consecutive days, and subsequent experiments were conducted 1 hour after the last administration. The xylene-induced mouse auricular swelling test was adopted, and ELISA was used to measure the serum tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2) and nitric oxide (NO) contents, so as to evaluate the anti-inflammatory activity and potential mechanisms of Pinellia ternata extract. The acetic acid-induced writhing test and the hot plate test were used to evaluate the anti-analgesic activity of Pinellia ternata extract. Results Pinellia ternata extract exhibited certain anti-inflammatory and analgesic activities. Compared with the control group, the ear edema, writhe counts, serum TNF-α, PGE2 and NO contents of mice in the medium- and high-dose Pinellia ternata extract groups decreased significantly (P<0.05); while the pain threshold prolonged significantly (P<0.05). With dosage of Pinellia ternata extract increasing, the auricular swelling inhibition rate and analgesic rate in mice increased progressively. Conclusion Pinellia ternata extract possesses certain anti-inflammatory and analgesic activities, and its anti-inflammatory mechanisms may be related to reducing serum TNF-α, PGE2 and NO contents.

关键词

半夏提取物 / 抗炎 / 镇痛 / 炎症介质 / 小鼠

Key words

Pinellia ternata extract / anti-inflammatory / analgesic / inflammatory mediators / mice

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基金资助

荆楚理工学院校级科研项目（ZKYB2402); 湖北省大学生科技创新训练计划项目（S202411336053; S202511336048）