Potential Application of Pseudomonas hunanensis MGJ–2 for Biodegradation of Nicotine in Tobacco Plants

摘要

Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues caused by nicotine have become increasingly severe.Traditional methods have proven ineffective in efficiently degrading residual nicotine.To address this issue,scientists both domestically and internationally have turned to biodegradation methods to tackle the environmental and health problems caused by residual nicotine.In this study,an enrichment method was used to screen bacteria with nicotine-degrading capabilities from the soil of tobacco planting sites at the Tobacco Research Institute of Heilongjiang in Bin County,Harbin City.Through phenotypic observations and 16S rDNA identification,a bacterial strain identified as Pseudomonas hunanensis MGJ–2 was isolated,capable of utilizing nicotine as a carbon and nitrogen source for growth.High-performance liquid chromatography （HPLC） analysis revealed that within 25 h,strain MGJ–2 could degrade nicotine 500 mg · L^-1 with an efficiency exceeding 99.9%.Strain MGJ–2 was applied to tobacco,and after 15 days of incubation and fermentation,it degraded 10.57% of nicotine in tobacco.Overall,the discovery of strain MGJ–2 enriched the resources of nicotine-degrading strains.Its remarkable biodegradation performance held immense potential for future biodegradation of nicotine in tobacco.

关键词

Pseudomonas hunanensis MGJ–2 / high-performance liquid chromatography(HPLC) / degradation characteristic / nicotine degradation