Cloning and Bioinformatics Analysis of CsFK111 Gene from Cucumbers

摘要

At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed geneCsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools wereused to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1 314 bp,which encoded 437 amino acids and was predicted tobe located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of thesecondary structure showed that β-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetictree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future studyon the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBKfamily proteins.

关键词

F-box gene / dwarf cucumber / gene cloning / bioinformatics analysis