Phylogenetic Analysis of Response of Sagittaria trifolia Linn. var. trifolia Genotypes of Northeast China to an Acetohydroxy Acid Synthase Inhibiting Herbicide

摘要

Sagittaria trifolia L.is a perennial aquatic herb that primarily reproduces clonally and through generative propagation.In recent years,S.trifolia has evolved a drastic resistance to acetohydroxy acid synthase （AHAS）-inhibiting herbicides in Northeast China.The phylogeographic patterns of S.trifolia with 31 purified resistance genotypes and five sensitive genotypes using chloroplast DNA （cpDNA） atpB–rbcL intergenic spacers were studied.Five haplotypes were characterized,and two of them were widely distributed in 36 genotypes.The dose response to bensulfuron-methyl showed that the GR₅₀ ranged from 2.07 g a.i.·hm^-2 to220.15 g a.i.·hm^-2.Sequencing of the AHAS gene indicated that 17 genotypes with the Pro197 mutation were distributed in haplotype 1,six genotypes with the Trp574 mutation were distributed in haplotype 3,and 13 genotypes with the wild AHAS gene were distributed in haplotypes 2,4 and 5.In the minimum-spanning network,the ancestral haplotypes 1 and 2 were widely distributed.Two primary clades were separated in the Bayes tree,and the result was consistent with the maximum likelihood tree.

关键词

Sagittaria trifolia L. / phylogeographic analysis / haplotype / bensulfuron-methyl resistance