Marginal Selenium Deficiency Alternates Inflammatory Response through NF-κB Pathway in LPS-induced Mouse Mastitis Model

摘要

The trace element selenium （Se） occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide （LPS） induced mouse mastitis model was established.Mice were fed with moderate Se diet (0.087 mg ·kg^-1 Se),adequate Se diet (0.15 mg ·kg^-1 Se) or excessive Se diet (1.5 mg ·kg^-1 Se) for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha （TNF–α）,interleukin–1β （IL–1β） and cyclooxygenase–2 （COX–2）;and enhanced the phosphorylation of NF–κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.

关键词

marginal / selenium deficiency / mastitis / inflammation / NF–κB signal pathway