外源茉莉酸甲酯对干旱胁迫下甘蓝生理特性及BoPRX基因表达的影响

Effects of exogenous methyl jasmonate on physiological characteristics and BoPRX gene expression of cabbage under drought stress

摘要

为探究茉莉酸甲酯（MeJA）对干旱胁迫下甘蓝幼苗的缓解效应和调节机制，以结球甘蓝秋甜二号幼苗为材料，设置 CK（正常生长管理）、T1（15% PEG8000 干旱胁迫）、T2（25 μmol·L^-1 MeJA 喷施后进行 15% PEG8000 干旱胁迫）3 种处理，测定各处理 0、3、6、12、24 h 的叶绿素含量、相对电导率（REC）、丙二醛（MDA）含量、抗氧化酶活性及过氧化物酶基因 PRX 表达量。结果表明，与 CK 相比，T1 处理的幼苗叶片叶绿素含量降低，而 REC 和 MDA 含量升高，超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT） （处理 24 h 除外）活性随着胁迫时间延长不断升高；而 T2 处理的叶绿素含量及 SOD、POD、CAT 活性较 T1 处理有所升高，REC、MDA 含量与 T1 处理相比有所降低。5 个 BoPRX 基因在 T1 和 T2 处理前期均迅速上调表达，在 12 h 或 24 h 后又下调表达，除 BoPRX113 外，其他 4 个 BoPRX 基因在 T2 处理下的表达水平低于 T1 处理。综上，外源 MeJA 可通过维持叶绿素含量、降低细胞膜损伤程度、调控抗氧化酶活性及 BoPRX 基因表达，缓解干旱胁迫对甘蓝幼苗的伤害，增强其抗旱性。本研究结果为甘蓝抗旱栽培提供了理论依据。

Abstract

To investigate the alleviation effect and relguatory mechanism of methyl jasmonate（MeJA）on cabbage seedlings under drought stress, the seedling of Qiu Tian 2 cabbage was used as experimental materials. Three treatments were established: CK（normal growth conditions）, T1（15% PEG8000- induced drought stress）, and T2（drought stress with 15% PEG8000 was applied after spraying with 25 μmol·L^-1 MeJA）. Chlorophyll content, relative electrical conductivity（REC）, malondialdehyde（MDA）content, antioxidant enzyme activity, and the expression patterns of peroxidase genes（PRX）were measured at 0, 3, 6, 12, and 24 h post-treatment. The results indicated that compared with CK, chlorophyll content in T1- treated seedlings decreased, while REC and MDA content increased. Activity of superoxide dismutase（SOD）, peroxidase（POD）, and catalase（CAT） （except treatment for 24 h）progressively increased over time under stress conditions. However, after T2 treatment, chlorophyll content, as well as the activity of SOD, POD, and CAT was higher than that in T1, while the REC and MDA content were reduced compared to the T1 treatment. The expression of the five BoPRX genes rapidly and were up- regulated expression in the early stages of both T1 and T2 treatments but were down-regulated expression after 12 or 24 h. Notably, except for BoPRX113, the expression level of other four BoPRX genes under T2 treatment were lower than those under T1 treatment. In conclusion, exogenous MeJA can alleviate the damage of drought stress to cabbage seedlings and enhance their drought resistance by maintaining chlorophyll content, reducing cell membrane damage, regulating the activity of antioxidant enzymes, and modulating the expression of BoPRX genes. The findings of this study provide a theoretical basis for drought-resistant cultivation of cabbage.

关键词

结球甘蓝 / MeJA / 干旱胁迫 / 生理效应 / PRX 基因

Key words

Cabbage / MeJA / Drought stress / Physiological effect / PRX gene

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基金资助

黑龙江省自然科学基金(PL2024C036)

黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155)

国家级大学生创新创业项目(202510232150X)