金钗石斛促生长内生真菌DN592对番茄生长与果实营养品质的影响

Effects of the growth-promoting endophytic fungus DN592 from Dendrobium nobile on tomato growth and fruit nutritional quality

摘要

目的 探讨中药材金钗石斛来源的内生真菌DN592对番茄生长及果实营养品质的影响,为药用植物内生真菌在农产品营养强化中的应用提供依据。方法 通过盆栽及田间试验,设置不同剂量DN592菌液处理,测定番茄株高、根鲜重等生长指标,以及果实甜度、番茄红素、维生素C等营养品质指标。结果 盆栽条件下,DN592处理均显著抑制番茄株高,同时根鲜重最大增幅达104.0%（P<0.05）；果实甜度、番茄红素和维生素C含量最高分别提升50.2%（P<0.05）、54.2%（P<0.05）和88.7%（P<0.001）,中剂量处理促根与提质效果更为均衡。田间试验中,低剂量DN592处理显著增加果实横径与纵径（P<0.05）,营养品质指标呈上升趋势但未达显著水平。结论 金钗石斛内生真菌DN592兼具控旺促根与提升番茄营养品质的双重效应,初步证实了药源内生菌转化应用于蔬菜生产的潜力,可为绿色微生物菌剂开发提供新的菌种资源。

Abstract

Objective This study aimed to investigate the effects of the endophytic fungus DN592, isolated from the Chinese medicinal plant Dendrobium nobile, on tomato growth and fruit nutritional quality, and to provide a basis for the application of medicinal plant-derived endophytic fungi in the nutritional biofortification of agricultural products. Methods Pot and field experiments were conducted with different doses of a DN592 fungal suspension. Growth indicators including plant height and root fresh weight were measured. In addition, fruit nutritional quality indicators such as sugar content, lycopene, and vitamin C were determined. Results Under pot conditions, all DN592 treatments significantly inhibited tomato plant height, while root fresh weight increased by up to 104.0% (P<0.05). The maximum increases in fruit sugar content, lycopene, and vitamin C were 50.2% (P<0.05), 54.2% (P<0.05), and 88.7% (P<0.001), respectively. The medium-dose treatment exhibited a more balanced effect on both root promotion and quality improvement. In the field experiment, the low-dose DN592 treatment significantly increased the transverse and longitudinal diameters of tomato fruits (P<0.05). The nutritional quality indicators showed an upward trend but did not reach significant levels. Conclusion The endophytic fungus DN592 from Dendrobium nobile demonstrates dual effects of suppressing excessive shoot growth and promoting root development while enhancing tomato fruit nutritional quality. This finding preliminarily confirms the potential of applying medicinal plant-derived endophytic fungi to vegetable production and provides new strain resources for the development of green microbial agents.

关键词

金钗石斛 / 内生真菌DN592 / 番茄 / 生长调控 / 果实营养品质

Key words

Dendrobium nobile / endophytic fungus DN592 / tomato / growth regulation / fruit nutritional quality

参考文献

[1] 翟笑,朱朝华,李欣荣,黄芪（Astragalus membranaceus (fisch.) bge.）种子内生细菌群落结构和功能[J].生态学杂志,2025,44（11):3578-3587.

[2] 姜超, 刘春燕, 曹旭, 等 . 植物内生菌多样性及其在作物生产中的作用机制研究进展[J]. 贵州农业科学, 2026, 54(1): 56-67.

[3] Hardoim P R, Van Overbeek L S, Berg G, et al. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes[J]. Microbiol Mol Biol Rev, 2015, 79(3): 293-320.

[4] Chauhan S, Kumari P, Deepa N, et al. Proteomic insights into plant-endophyte interactions: advancing understanding of mutualistic symbiosis and plant resilience[J]. Crit Rev Biotechnol, 2026, 46(3): 440-459.

[5] 阙彩霞, 杨燕妮, 王再花, 等 . 金钗石斛不同开花时期挥发性物质成分与超微结构特征分析[J/OL]. 广西植物, 1-14[2026-06-16]. https://link.cnki.net/urlid/45.1134.Q.20260224.1254.002.

[6] 蒋勇炫, 毛黔华, 林夏萍, 等 . 金钗石斛生物碱对对乙酰氨基酚诱导小鼠急性肝损伤的保护作用[J]. 遵义医科大学学报, 2024, 47(11): 1047-1053.

[7] 张逸群, 陈家栋, 李梦妮, 等 . 药用植物铁皮石斛内生菌多样性研究进展[J]. 浙江农业科学, 2025, 66(7): 1627-1631.

[8] 童文君 . 美花石斛内生菌多样性分析及促生潜力研究[D]. 南京： 南京师范大学, 2014.

[9] 张敏, 张丽莉, 王虹云, 等 . 植物内生菌及其在番茄生长发育中的作用研究进展[J]. 中国瓜菜, 2025, 38(7): 10-16.

[10] 杨洪一,

[11] Oumar A A, 郭普悦, 等 . 植物内生菌抗重金属胁迫及土壤修复研究进展[J]. 现代农业科技, 2026（8): 94-97.

[12] 马莹, 曹梦圆, 石孝均, 等 . 植物促生菌的功能及在可持续农业中的应用[J]. 土壤学报, 2023, 60(6): 1555-1568.

[13] Ruiz K B, Novello G, Tejos R, et al. Exploring quinoa's plant growth-promoting bacteria: a pseudomonas seed endophyte promotes salinity tolerance and modifies root architecture in arabidopsis thaliana[J]. Plant Stress, 2026, 21: 101371.

[14] 张静怡, 赵龙飞, 刘梦洁 . 植物内生菌多样性、功能及应用研究进展[J]. 微生物学报, 2025, 65(4): 1446-1468.

[15] 翟凯辉, 张影影, 高夕全 . 种子内生菌促生机制和抗病机理研究进展[J]. 农业生物技术学报, 2023, 31(9): 1965-1979.

[16] Li L, Liu C, Wen W, et al. Dendrobine biosynthesis in dendrobium nobile in four different habitats is affected by the variations in the endophytic fungal community[J]. Front Microbiol, 2022, 13: 981070.

[17] Chen W, Sun Q, Wang J, et al. Colonization by the endophytic fungus phyllosticta fallopiae combined with the element Si promotes the growth of dendrobium nobile[J]. Int J Biol Macromol, 2024, 274(Pt 2): 133343.

[18] Chen W, Wang J, Song J, et al. Exogenous and endophytic fungal communities of dendrobium nobile lindl. across different habitats and their enhancement of host plants' dendrobine content and biomass accumulation[J]. ACS Omega, 2023, 8(13): 12489-12500.

[19] Shah S, Paudel M R, Thapa B B, et al. Extract from endophytic fusarium isolates stimulates seed germination of the host and protocorm development of non-host orchids[J]. Commun Integr Biol, 2024, 18(1): 2439798.

[20] 黎艳, 孙孟园, 蒋亨珂, 等 . 类受体激酶调控根生长发育的研究进展[J]. 西北植物学报, 2018, 38(12): 2336-2346.

[21] 姜卉, 王小龙, 王守创, 等 . 番茄风味物质代谢途径解析与分子育种研究进展[J]. 生物技术通报, 2026, 42(3): 60-78.

[22] 李君明, 项朝阳, 王孝宣, 等 . “十三五”我国番茄产业现状及展望[J]. 中国蔬菜, 2021（2): 13-20.

[23] 牛艳, 王晓静, 陈翔, 等 . 中国番茄产业发展的现状问题和对策及宁夏番茄产业发展成效[J]. 黑龙江农业科学, 2022, 45(12): 70-74.

[24] 李晴, 柴霜, 冯千禧, 等 . 植物内生菌在农作物方面的应用探析[J]. 种子科技, 2021, 39(12): 8-9.

[25] 冯燕辉, 熊娟, 黎烨, 等 . 接种2种不同促生菌对番茄根菌群结构及多样性的影响[J]. 福建农业科技, 2021, 52( 6): 6-14.

[26] 徐云燕, 刘杰, 刘波 . 金钗石斛生物碱对小鼠肠道菌群的作用[J]. 遵义医科大学学报, 2025, 48(9): 889-895.

[27] 伍善东, 雷平, 郭照辉, 等 . 1株番茄青枯病内生拮抗细菌的分离鉴定及盆栽防效[J]. 湖南农业大学学报（自然科学版）, 2016, 42(6): 627-630.

[28] 刘玉婷, 郭静, 张重义, 等 . 根际微生态视角下的中药材道地性研究进展[J]. 中国现代中药, 2025, 27(8): 1594-1602.

[29] 李肇高 . 内生真菌共培养金钗石斛合成石斛碱的技术体系构建及分子机制分析[D]. 遵义： 遵义医科大学, 2024.

[30] 范广斌, 岳万勇, 李梅, 等 . 不同营养液灌溉和栽培密度对番茄生长和品质的影响[J]. 种子科技, 2026, 44(5): 32-34.

[31] Pieterse C M, Zamioudis C, Berendsen R L, et al. Induced systemic resistance by beneficial microbes[J]. Annu Rev Phytopathol, 2014, 52: 347-375.

[32] 刁风伟, 黄琰哲, 许子荣, 等 . 根际细菌对番茄幼苗生长的影响[J]. 安徽农业科学, 2024, 52(11): 32-35.

[33] Shoresh M, Harman G E, Mastouri F. Induced systemic resistance and plant responses to fungal biocontrol agents[J]. Annu Rev Phytopathol, 2010, 48: 21-43.

[34] Cosner J, Pandharikar G, Tremble K, et al. Fungal endophytes[J]. Curr Biol, 2025, 35(19): 904-910.

[35] Islam R. Protective fusarium spp. in plant growth promotion: a novel approach[J]. Eur J Biotech Biosci, 2025, 13(1): 16-22.

[36] Contreras-Cornejo H A, Macías-Rodríguez L, Cortés-Penagos C, et al. A plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in arabidopsis[J]. Plant Physiol, 2009, 149(3): 1579-1592.

[37] Huot B, Yao J, Montgomery B L, et al. Growth-defense tradeoffs in plants: a balancing act to optimize fitness[J]. Mol Plant, 2014, 7(8): 1267-1287.

[38] 张国显, 范永怀, 赵凤艳, 等 . 化肥减量配施有机物料对设施番茄生长、光合特性、产量及品质的影响[J]. 中国科技论文, 2018, 13(6): 698-703.

[39] 李博, 张曼, 熊正琴 . 施用氮肥与生物炭对菜地净综合温室效应的影响[J]. 中国科技论文, 2014, 9（9): 1057-1062.

[40] 连青龙, 张跃峰, 丁小明, 等 . 我国北方设施蔬菜质量安全现状与问题分析[J]. 中国蔬菜, 2016, 20(7): 15-21.

[41] 王艳, 银洁, 田雪飞, 等 . 中医药助力健康中国战略的价值与实践路径探析[J]. 湖南中医药大学学报, 2023, 43(7): 1240-1244.

[42] 赵佳璇, 王建 . 习近平健康中国观的发展脉络、核心内涵及实践路径——以《习近平关于健康中国论述摘编》为考察视角[J]. 卫生法学, 2026, 34(2): 23-32.

[43] 王锐竹, 王丽, 田猛, 等 . 熊蜂授粉对设施番茄产量、品质及效益的影响[J]. 天津农林科技, 2018, 35(2): 5-6.

[44] 鲁凯珩, 金清, 曹沁, 等 . 不同微生物菌剂对田间西红柿品质以及土壤酶活性的影响[J]. 上海师范大学学报（自然科学版）, 2019, 48(2): 197-206.

[45] 王锐竹, 张艳玲, 于艳洁, 等 . AM真菌对番茄产量品质及根区环境的影响[J]. 天津农林科技, 2016（2): 22-23.

[46] 张鹏.茄科植物番茄红素联合盐酸坦索罗辛治疗BPH及LUTS的临床疗效及安全性评价[J].分子植物育种,2023,21（8):2710-2714.

[47] 孟凯丽, 孙世东 . 番茄红素生物活性及其淀粉基复合稳定制剂研究进展[J]. 粮油科学与工程, 2025, 39(6): 17-22.

[48] Imran M, Ghorat F, Ul-Haq I, et al. Lycopene as a natural antioxidant used to prevent human health disorders[J]. Antioxidants (Basel), 2020, 9(8): 706.

[49] Rowland A, Edwards S, Prieto-Bellver G, et al. A comprehensive review of the physiology and evidence base to guide the use of ergogenic and medical supplements for enhanced cycling performance[J]. J Int Soc Sports Nutr, 2026, 23(1): 2630487.

[50] Ibiang S R, Sakamoto K. Modulation of phytochemicals and essential trace elements in fruits of different tomato cultivars by the endophytic fungus penicillium pinophilum EU0013s[J]. Microbes Environ, 2022, 37(3): ME22026.

[51] Negwer E J, Bilger W. Kinetics of caffeoylquinic acid formation in N-deficient sunflower as a test of the growth-defense trade-off framework[J]. J Exp Bot, 2026, 77(4): 1309-1323.

[52] Yu Y, Gui Y, Li Z, et al. Induced systemic resistance for improving plant immunity by beneficial microbes[J]. Plants (Basel), 2022, 11(3): 386.

[53] 宋经元, 郭顺星 . 离体培养时真菌对铁皮石斛和金钗石斛生长的影响[J]. 中国医学科学院学报, 2001, 23(6): 547-551.

[54] 王位, 薛鸣, 雷婷越, 等 . 番茄青枯病拮抗菌的筛选及防治效果[J]. 海南大学学报（自然科学版）, 2024, 42(1): 30-36.

[55] Sanchez-Contreras M, Bauer W D, Gao M, et al. Quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes[J]. Philos Trans R Soc Lond B Biol Sci, 2007, 362(1483): 1149-1163.

[56] Götz-Rösch C, Sieper T, Fekete A, et al. Influence of bacterial N-acyl-homoserine lactones on growth parameters, pigments, antioxidative capacities and the xenobiotic phase II detoxification enzymes in barley and yam bean[J]. Front Plant Sci, 2015, 6: 205.

[57] 赵卫松, 李社增, 鹿秀云, 等 . 解淀粉芽胞杆菌PHODB35对小麦的促生作用及产量影响[J]. 中国生物防治学报, 2019, 35(3): 399-406.

基金资助

国家自然科学基金资助项目(31960074)

教育部供需对接就业育人项目(2025030310008)

教育部产学合作协同育人项目(230901414190927)