脂代谢相关基因ABHD5抑制肾癌细胞体外迁移及侵袭

Lipid metabolism-related gene ABHD5 inhibits renal cancer cell migration and invasion in vitro

摘要

目的 探讨透明细胞肾细胞癌（clear cell renal cell cancinoma，ccRcc）细胞体外迁移及侵袭过程中与α/β自水解酶结构域５（α/β-hydrolase domain-containing 5，ABHD5）的关系。 方法 采用TCGA数据库分析人ccRcc组织及正常肾组织中ABHD5的表达情况。采用ABHD5过表达慢病毒转染人肾透明细胞癌细胞株786-O及Caki-1后，运用real-time PCR及Western blot技术检测ABHD5表达改变情况。采用Transwell实验、划痕实验检测此基因对细胞体外迁移及侵袭能力的影响。 结果 TCGA数据库显示ccRcc组织中ABHD5表达较正常肾脏组织显著下降（P=0.000），且其下降程度在复发转移肿瘤中进一步增加（P=0.007），与患者生存呈显著负相关（P<0.001）。Transwell实验、划痕实验显示过表达ABHD5显著抑制肾癌细胞体外迁移及侵袭能力（P<0.05）。过表达ABHD5通过影响Sting信号进而影响肾癌细胞体外迁移及侵袭。 结论 脂代谢相关基因ABHD5抑制ccRcc癌细胞体外迁移及侵袭能力。

Abstract

Objective To investigated the relationship between clear cell renal cell carcinoma（ccRcc） cells and α/β-hydrolase domain-containing 5（ABHD5） during migration and invasion in vitro. Methods The TCGA database was used to analyze the expression of ABHD5 in human ccRcc tissues and normal kidney tissues. After transfection of human ccRcc cell lines 786-O and Caki-1 with ABHD5-overexpressing lentivirus， the changes in ABHD5 expression were detected using real-time PCR and Western blot. Transwell assay and scratch assay were used to examine the effects of ABHD5 on cell migration and invasion in vitro. Results TCGA database showed that the expression of ABHD5 in ccRcc tissues was significantly decreased compared with normal renal tissues（P=0.000）， and the decrease was greater in recurrent metastatic tumors（P=0.007），which was significantly negatively correlated with the survival of the patients（P<0.001）. Transwell assay and scratch assay showed that ABHD5 overexpression significantly inhibited the migration and invasion of ccRcc cells in vitro（P<0.05）. Overexpression of ABHD5 inhibited renal cancer cell migration and invasion in vitro by influencing the Sting signaling pathway. Conclusion Lipid metabolism-related gene ABHD5 inhibited the migration and invasion of ccRcc cells in vitro.

关键词

透明细胞肾细胞癌 / 脂代谢 / α/β-水解酶结构域5 / 迁移 / 侵袭

Key words

clear cell renal cell carcinoma / lipid metabolism / α/β-hydrolase domain-containing 5 / migration / invasion

基金资助

重庆市渝北区科委重点课题资助项目(2021（农社）38)