质谱流式成像技术及其在肿瘤研究中的应用进展

黄一薇; 向廷秀; 刘星河; 冉静; 赵毅

doi:10.13406/j.cnki.cyxb.003639

重庆医科大学学报 ›› 2025, Vol. 50 ›› Issue (01) : 6 -13. DOI: 10.13406/j.cnki.cyxb.003639

综述

质谱流式成像技术及其在肿瘤研究中的应用进展

黄一薇 ¹ ,
向廷秀 ¹ ,
刘星河 ² ,
冉静 ¹ ,
赵毅 ¹

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Imaging mass cytometry technology and its application in tumor research

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摘要

多重单细胞蛋白组技术已成为生物医学研究的热点，其中质谱流式成像（imaging mass cytometry，IMC）彻底解决了荧光基团间严重的串色问题，并弥补了单细胞测序技术缺失的组织空间信息。该技术能够在单张组织切片上同时标记数十种靶标，并在单细胞层面获得它们的表达水平及细胞定位，进行深度的细胞表型原位分析并从时间和空间水平直观描绘出单细胞蛋白组图谱，因其独特的技术优势逐渐成为肿瘤研究领域新兴的强有力工具。本文将详细介绍IMC的技术原理，并通过分析近期应用案例阐述其在细胞表型鉴定、生物标志物检测、肿瘤免疫调节判定、肿瘤异质性区分、临床预后指导及反应预测等肿瘤研究方面的应用进展，推动肿瘤空间组学与现有技术的进一步结合。

Abstract

Multiplex single-cell proteomics technology has become a hot topic in biomedical research，among which imaging mass cytometry（IMC） completely solves the serious problem of cross-color between fluorophores and makes up for the lack of tissue spatial information in single-cell sequencing technology. This technique can label dozens of targets simultaneously on a single tissue section，obtain their expression levels and cell localization at the single-cell level，perform in-depth cell phenotypic in situ analysis，and visually depict single-cell proteome maps from the temporal and spatial levels，and due to its unique technical advantages，it has become a powerful tool in the field of tumor research. This article elaborates on the technical principle of IMC and summarizes the advances in the application of IMC in cell phenotype identification，biomarker detection，tumor immunomodulatory determination，tumor heterogeneity differentiation，clinical prognosis guidance，and response prediction by analyzing recent cases，so as to promote the further integration of tumor spatial omics with existing technologies.