百里香酚通过SCIN改善自闭症核心症状的作用研究

王楚萱; 余丽娟; 吕明其; 李英博

doi:10.13406/j.cnki.cyxb.003850

重庆医科大学学报 ›› 2025, Vol. 50 ›› Issue (07) : 957 -962. DOI: 10.13406/j.cnki.cyxb.003850

基础研究

百里香酚通过SCIN改善自闭症核心症状的作用研究

王楚萱 ¹ ,
余丽娟 ² ,
吕明其 ² ,
李英博 ¹

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Thymol improves the core symptoms of autism through regulating scinderin expression

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摘要

目的：通过构建丙戊酸（valproic acid,VPA）诱导的自闭症（autism spectrum disorder,ASD）大鼠模型并进行百里香酚（thymol,Thy）的干预，探讨Thy通过肌切蛋白（scinderin,SCIN）改善ASD大鼠核心症状的作用。方法：通过三箱社交实验、旷场实验以及青年社交实验观察大鼠的ASD样行为；RNA-seq实验检测大鼠前额叶皮层（prefrontal cortex,PFC）中RNA表达情况；Western blot实验检测大鼠PFC中SCIN蛋白水平。结果：与对照组大鼠相比，模型组大鼠表现出了ASD样行为，包括社交能力下降、社交偏好受损、探索能力下降、重复刻板行为[与对照组大鼠相比，模型组大鼠站立次数减少（P<0.001）；自我梳理时间增加，（P=0.003）]等，且PFC中SCIN表达水平升高；通过对模型组大鼠进行Thy治疗后，其ASD样行为明显改善[（与模型组大鼠相比，干预组大鼠站立次数增加（P<0.001）；自我梳理时间减少（P=0.004）]，且SCIN表达基本恢复到了正常水平。结论：ASD模型大鼠PFC中SCIN的异常表达可能会导致大鼠的ASD样行为，Thy干预后可以降低SCIN的表达水平从而改善ASD核心症状。

Abstract

Objective To explore the effect of thymol on improving the core symptoms of autism through regulating scinderin expression in autism spectrum disorder（ASD） rats by constructing valproic acid-induced ASD rat model and intervening with thymol. Methods The ASD-like behaviors of rats were observed using three box social experiment，open field experiment，and youth social experiment. RNA-seq was used to detect the expression of RNA in the prefrontal cortex of rats. The protein level of scinderin in the prefrontal cortex of rats was measured by Western blot. Results Compared with the rats in the control group，the rats in the model group showed ASD-like behaviors，including decreased social ability，impaired social preference，decreased exploration ability，and repeated stereotyped behavior. Compared with the control group，the model group showed reduced standing times（P<0.001） and increased self combing time（P=0.003）. Moreover，the expression level of scinderin in the prefrontal cortex increased in model rats. After thymol treatment，the ASD-like behaviors in the model group were significantly improved. Compared with the model group，the number of standing times in the intervention group increased（P<0.001） and the self grooming time decreased（P=0.004）. The expression of scinderin basically returned to the normal level. Conclusion The abnormal expression of scinderin in the prefrontal cortex of ASD model rats can lead to ASD-like behaviors. Thymol intervention can reduce the expression of scinderin and improve the core symptoms of ASD.