全基因组水平的小麦渍水、高温及其复合胁迫响应因子鉴定

孙龙清; 秦丹丹; 董静; 郑兴飞; 程红娜; 付大龙; 雷添杰; 徐得泽

doi:10.13928/j.cnki.wrahe.2025.10.019

水利水电技术（中英文） ›› 2025, Vol. 56 ›› Issue (10) : 243 -256. DOI: 10.13928/j.cnki.wrahe.2025.10.019

农村水利

全基因组水平的小麦渍水、高温及其复合胁迫响应因子鉴定

孙龙清 ¹^,²^,³ ,
秦丹丹 ¹^,²^,³ ,
董静 ¹^,²^,³ ,
郑兴飞 ¹^,²^,³ ,
程红娜 ¹^,²^,³^,⁴ ,
付大龙 ¹^,²^,³ ,
雷添杰 ⁵ ,
徐得泽 ¹^,²^,³

作者信息 +

Waterlogging, high temperature and composite stress responsive transcriptome analysis of wheat

Longqing SUN ¹^,²^,³ ,
Dandan QIN ¹^,²^,³ ,
Jing DONG ¹^,²^,³ ,
Xingfei ZHENG ¹^,²^,³ ,
Hongna CHENG ¹^,²^,³^,⁴ ,
Dalong FU ¹^,²^,³ ,
Tianjie LEI ⁵ ,
Deze XU ¹^,²^,³

Author information +

文章历史 +

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摘要

【目的】随着全球气候变化加剧，高温和渍水已成为影响小麦生产的主要非生物胁迫因子。解析小麦响应上述胁迫的关键代谢通路和调控网络，将为小麦抗逆育种提供理论依据。【方法】以小麦品种鄂麦007为材料，在三叶一心期分别进行24 h的单一高温(30℃)、单一渍水及二者复合胁迫处理，以未处理植株为对照，利用RNA-seq技术对叶片进行转录组测序，通过DESeq2分析差异表达基因，并进行GO功能注释和KEGG通路富集分析，通过qRT-PCR验证关键基因的表达模式。【结果】共鉴定到30 648个差异基因，渍水、高温、渍水+高温胁迫下分别鉴定到3 088个、13 291个和11 269个差异表达基因。其中，902个基因在三种胁迫中均差异表达。GO和KEGG富集分析表明，902个共同响应基因主要参与了烟酰胺代谢、半胱氨酸和蛋氨酸代谢等通路。【结论】高温和渍水胁迫通过协同抑制烟酰胺代谢和半胱氨酸代谢通路，破坏铁离子稳态并削弱抗氧化能力，从而加剧小麦的氧化损伤，复合胁迫的效应显著强于单一胁迫，这为解析小麦抗逆机制提供了新视角，为分子设计育种提供理论支撑，对培育适应气候变化的抗逆小麦新品种具有重要实践价值。

Abstract

[Objective] Under climate change scenarios, high temperature and waterlogging stresses have emerged as critical constraints for wheat production. Elucidating the molecular mechanisms of stresses resistance in wheat, with particular emphasis on identifying key metabolic pathways and regulatory networks underlying these stresses tolerance, will provide theoretical basis for molecular breeding.[Methods] 24 hours' single high temperature(30 ℃), single waterlogging and compound stress treatments were conducted on the wheat variety Emai 007 at the three-leaf one-center stage. Basing on RNA-seq, transcriptome analysis on leaves of Emai 007 under the three treatments was performed, and differentially expressed genes(DEGs) were analyzed by DESeq2, and GO and KEGG pathway enrichment analysis were performed. [Results] Transcriptome profiling identified 30 648 DEGs, including 3 088, 13 291, and 11 269 DEGs responsive to waterlogging, high temperature, and combined stress, respectively. Notably, 902 core DEGs were consistently regulated across all the stress treatments. GO and KEGG enrichment analysis revealed that these core DEGs were predominantly enriched in nicotinamide metabolism and sulfur-containing amino acid(cysteine and methionine) metabolic pathways. [Conclusion] It was demonstrated that the combined stress synergistically exacerbates oxidative damage through coordinated suppression of nicotinamide and sulfur metabolic pathways, leading to disrupted iron homeostasis and compromised antioxidant capacity. The identification of these conserved stress-responsive pathways provides novel insights into adaptation mechanisms of wheat to concurrent abiotic stresses. Furthermore, this study offers valuable theoretical foundations for developing climate-resilient wheat varieties through molecular breeding approaches.