灰杨 PcAHL15 正调控拟南芥的耐盐性

Populus × canescens PcAHL15 positively regulates salt stress tolerance in Arabidopsis thaliana

摘要

【目的】探究灰杨 AT-hook 基序核定位蛋白 PcAHL15 在响应盐胁迫中的功能，为杨树抗逆遗传改良提供理论依据。【方法】从灰杨中克隆了 PcAHL15 基因，并对其进行系统进化分析；通过亚细胞定位和 RT-qPCR 技术检测其表达模式；在拟南芥中异源过表达 PcAHL15，并在盐胁迫条件下比较过表达植株与野生型、转空载体植株的表型差异，测定其形态指标、生物量以及生理指标。【结果】（1）系统进化分析表明，灰杨 PcAHL15 与银白杨和毛果杨中的同源蛋白亲缘关系最近。（2）盐胁迫处理下，灰杨根、茎、叶中 PcAHL15 基因的表达水平均发生显著变化。（3）与野生型和转空载体对照相比，过表达 PcAHL15 的拟南芥株系在盐胁迫下表现出更强的耐受性，具体表现为：叶片黄化萎蔫程度减轻、根长与种子萌发率显著增加、细胞膜受损程度降低以及钠离子（Na⁺）积累量减少。【结论】灰杨 PcAHL15 基因在植物盐胁迫应答中发挥正调控作用，其过表达能显著增强拟南芥的耐盐性。

Abstract

[Objective] This study aims to elucidate the function of Populus × canescens AT-hook motif nuclear localization protein PcAHL15 in plant salt stress response, so as to provide a theoretical basis for the genetic improvement of stress resistance in poplar. [Method] The PcAHL15 gene was cloned from P. × canescens; phylogenetic analysis of PcAHL15 was conducted; the expression pattern of PcAHL15 was detected via subcellular localization and RT-qPCR; PcAHL15 was heterologously expressed in Arabidopsis thaliana, and under salt stress, phenotypic identification was performed on transgenic lines, with morphological indices, biomass accumulation, and physiological indicators measured. [Result] (1) P. × canescens PcAHL15 is most closely related to P. alba and P. trichocarpa. (2) Under salt stress, the expression level of PcAHL15 in the roots, stems, and leaves of P. × canescens was significantly altered. (3) Compared with wild-type and empty vector-transformed lines, the PcAHL15-overexpressing lines exhibited alleviated leaf yellowing and wilting, increased root length, higher seed germination rate, reduced cell membrane damage, and decreased Na⁺ accumulation. [Conclusion] Populus × canescens PcAHL15 positively regulates plant salt stress responses, and its overexpression confers a significant enhancement of salt tolerance in Arabidopsis.

关键词

灰杨 / 盐胁迫 / 离子平衡 / 活性氧 / AT-hook 核定位蛋白

Key words

Populus × canescens / salt stress / ion balance / reactive oxygen species / AT-hook nuclear localization protein

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基金资助

国家自然科学基金项目(32371828)

国家自然科学基金项目(32071730)