食窦魏斯氏菌 HDE-10 胞外多糖的结构及理化性质

Structure and Physicochemical Properties of Exopolysaccharides From Weissella cibaria HDE-10

摘要

本研究从自然酸菜发酵液中分离筛选出一株产胞外多糖(Exopolysaccharides,EPS)的乳酸菌(Lacticacid bacteria,LAB)，经16SrDNA序列比对，鉴定为食窦魏斯氏菌(Weissellacibaria)，编号为HDE-10.以蔗糖为底物，利用该菌株发酵生产EPS，再经分离提纯获得高纯度EPS.结构与性质分析表明，该EPS是由α-(1,6)糖苷键连接的葡聚糖，分子量为1.878×10⁶Da，呈无定形片状结构.溶解度为(95.98±5.56)%，持水量为(424.43±5.52)%，黏度较高，对大豆油、汽油和苯均有较好的乳化作用.本研究扩展了产EPS乳酸菌的来源，并为乳酸菌EPS的功能特性及其应用提供了理论支持.

Abstract

A strain of lactic acid bacteria (LAB) producing exopolysaccharides (EPS) was isolated and screened from the fermentation broth of natural sauerkraut. After 16S rDNA sequence alignment, it was identified as Weissella cibaria, named HDE-10. EPS was produced by strain fermentation using sucrose as substrate, and then high purity EPS was obtained by separation and purification. The structure and physical properties analysis showed that the EPS was a glucan composed of α-(1,6) glycosidic bonds, with a molecular weight of 1.878×10⁶ Da and an amorphous sheet structure. The solubility was (95.98±5.56)%, the water holding capacity was (424.43±5.52)%, the viscosity was high, and it had good emulsification effect on soybean oil, gasoline and benzene. This study expanded the source of EPS-producing lactic acid bacteria and provided theoretical support for the functional characteristics and application of EPS produced by lactic acid bacteria.

关键词

食窦魏斯氏菌 / 胞外多糖 / 鉴定 / 结构 / 特性

Key words

Weissella cibaria / exopolysaccharides / identification / structure / characteristic

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基金资助

黑龙江省自然科学基金项目(PL2025C077)

优秀青年教师基础研究支持计划项目(YQJH2024199)

大学生创新创业训练计划项目(S202610212049)