下丘脑室旁核内GABA_A受体活性对小鼠焦虑样行为的影响

Effects of GABA_A Receptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice

摘要

目的 观察下丘脑室旁核（PVN）内GABA_A受体活性对小鼠焦虑样行为的影响。方法 随机选取3只正常KM小鼠,荧光免疫组织化学法观察下丘脑PVN内GABA_A受体表达;随机选取24只正常KM小鼠,按下丘脑PVN内注射不同药物分为生理盐水（NS）组（0.3 μL）、GABA_A受体激动剂Isoguvacine（Isog,0.3 μL）组或GABA_A受体拮抗剂Bicuculline（BIC,0.3 μL）组,每组8只。观察各组小鼠在PVN微量注射不同药物后的焦虑样行为变化。结果 小鼠下丘脑PVN内有GABA_A受体表达;旷场实验、高十字架迷宫实验、埋珠实验结果均显示,PVN内微量注射Isog,小鼠没有出现焦虑样行为（P>0.05）,而PVN内微量注射BIC,促进了小鼠的焦虑状态（P<0.05）。结论 小鼠PVN内的GABA_A受体活性可能与抗焦虑作用有关。

Abstract

Objective To observe the effects of GABA_A receptor in the paraventricular nucleus (PVN) on anxiety-like behavior in mice. Methods Three normal KM mice were randomly selected to observe the expression of GABA_A receptors in the PVN using fluorescence immunohistochemistry. Additionally, twenty-four normal KM mice were randomly selected and divided into three groups based on the microinjection of different drugs into the PVN: Normal saline (NS) group (0.3 μL), GABA_A receptor agonist Isoguvacine (Isog) group (0.3 μL), and GABA_A receptor antagonist Bicuculline (BIC) group (0.3 μL), with 8 mice in each group. The anxiety-like behavior changes of the mice after microinjection of different drugs in the PVN were observed. Results GABA_A receptors were expressed in the PVN of mice. Results from open field, elevated plus maze, and marble burying tests showed that microinjection of GABA_A receptor agonist into the PVN did not induce anxiety-like behavior in mice (P>0.05), while microinjection of a GABA_A receptor antagonist promoted anxiety-like behavior (P<0.05). Conclusion The activity of GABA_A receptors within the PVN may be associated with anxiolytic effects in mice.

关键词

下丘脑室旁核 / GABA_A受体 / 焦虑

Key words

Periventricular nucleus of the hypothalamus / GABA_A receptors / Anxiety

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