一氧化氮供体型药物对牙龈卟啉单胞菌体外抗菌活性的影响

Effect of nitric oxide donor drugs on the antibacterial activity of Porphyromonas gingivalis in vitro

摘要

目的  研究一氧化氮（nitric oxide，NO）供体型药物对牙龈卟啉单胞菌（Porphyromonas gingivalis，P.g）体外抗菌活性的影响。方法  采用菌落计数法、总NO检测试剂盒、活性氧检测试剂盒、钙离子（Ca²⁺）荧光探针Fluo-4 AM检测不同浓度的NO（10、30、50 mmol/L）对P.g的体外抗菌性能，以及对P.g胞内活性氧（reactive oxygen species，ROS）水平和Ca²⁺浓度的影响。结果  NO对P.g有显著的体外抗菌活性，并且其抗菌活性与NO药物浓度和作用时间具有明显的正相关性(P<0.05)。NO处理后显著提高了P.g胞内ROS水平和Ca²⁺浓度(P<0.05)。结论  NO能够有效抑制P.g的活性和生长能力，并引起P.g胞内ROS和Ca²⁺代谢紊乱。NO供体型药物对种植体周围炎具有潜在的治疗作用和临床应用价值。

Abstract

Objective  To study the effect of nitric oxide （NO） on the in vitro antibacterial activity of Porphyromonas gingivalis (P.g). Methods  The antibacterial performance of different concentrations of NO (10,30,50 mmol/L) on P.g was assessed by colony forming units,total NO detection kit,reactive oxygen species detection kit,and calcium ion fluorescent probe Fluo-4 AM. Moreover,the effects of NO treatment on intracellular levels of reactive oxygen species （ROS） and calcium ions in P.g cells were also examined.  Results  NO exhibited significant in vitro antibacterial activity against in P.g cells,and its antibacterial activity was positively correlated with the concentration of NO drugs and treatment duration (P<0.05). NO treatment significantly increased intracellular levels of ROS and calcium ions in P.g cells (P<0.05). Conclusion  NO can effectively inhibit the activity and growth ability of P.g,and cause intracellular ROS and calcium ion metabolism disorders. Our study demonstrates the potential therapeutic effect and clinical application of NO donor drugs in peri-implantitis.

关键词

一氧化氮供体型药物 / 牙龈卟啉单胞菌 / 抗菌作用 / 种植体周围炎 / 活性氧 / 钙离子

Key words

nitric oxide donor drugs / Porphyromonas gingivalis (P.g) / antimicrobial activity / peri-implantitis / reactive oxygen species / Ca²⁺