反转学习行为学范式对Sapap3 基因敲除小鼠认知灵活性的检测

Detection and exploration of cognitive flexibility by reversal learning

摘要

目的  训练C57/6J野生型小鼠与Sapap3基因敲除小鼠分别进行嗅觉、触觉、听觉为线索的反转学习行为学学习，探究嗅觉、触觉、听觉为线索的小鼠反转学习范式的可行性与对Sapap3基因敲除鼠认知灵活性的探究。方法  将8~10周的C57/6J野生型小鼠与Sapap3基因敲除鼠各分成3组，在头部固定的情况下进行反转学习行为学训练，通过分析各个阶段的舔水事件的正确率、错误率与学习速率等指标，观察小鼠在3种反转学习行为学范式下的学习能力。结果  Sapap3基因敲除鼠与C57/6J野生型小鼠在以嗅觉与听觉为线索的反转学习范式反转阶段达到学习标准所需的时间差异有统计学意义。结论  反转学习行为学范式可用于对认知灵活性的检测并且Sapap3基因敲除小鼠的认知灵活性存在障碍，为后续进行对与认知灵活性相关的疾病与脑区的研究的奠定了行为学基础。

Abstract

Objective  To train C57/6J wild-type mice and Sapap3 gene knockout mice to perform reversal learning induced by olfaction, tactility audition, respectively, and to explore the feasibility of reversal learning paradigm induced by olfaction, tactility audition and the cognitive flexibility of Sapap3 gene knockout mice. Methods  C57/6J wild-type mice and Sapap3 gene knockout mice (8-10 weeks old) were randomly divided into three groups and trained with the head fixed. The correct rate, error rate and learning rate of the licking events at each stage were analyzed to observe the learning ability of the mice under the three reversal learning paradigms. Results  The time required to reach the learning criterion was significantly different between Sapap3 knockout mice and C57/6J wild type mice in the reversal stage of olfactory and auditory reversal learning paradigm. Conclusion  The behavioral paradigm of reversal learning can be used to detect cognitive flexibility, which lays a behavioral foundation for the subsequent research on the diseases and brain regions related to cognitive flexibility.

关键词

认知灵活性 / 反转学习 / 强迫症

Key words

cognitive flexibility / reversal learning / obsessive compulsive disorder