海州湾细菌群落结构多样性及环境因子分析

Diversity of Bacterial Community Structure and Environmental Factors in Haizhou Bay

摘要

为探究海州湾浅海域的细菌群落结构多样性及其影响因素, 选取海州湾6个点位的表层水和底层水样品, 基于高通量测序技术, 对海州湾浅海域细菌群落结构多样性及其分布特征与环境因子的关系进行了分析。实验结果表明, 底层水样的细菌群落丰度和多样性优于表层水样, 二者细菌群落间的进化方向具有一定的差异, 但二者的细菌群落间差异不显著; 海州湾水域共发现2 491个操作分类单元(operational taxonomic unit, OTU), 分别属于32个门、74个纲、116个目、200个科和364个属; 在门水平上, 海州湾水域的主要优势菌群为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和蓝细菌门(Cyanobacteria)等; 铵盐(ammonium, NH4-N)和叶绿素(chlorophyll, Chl)是影响海州湾细菌群落结构的主要环境因子。本研究证实海州湾浅海域细菌群落结构多样性分布与环境因子有一定的关联性, 为海州湾浅海域生态系统的可持续发展提供了理论依据。

Abstract

In order to explore the diversity of bacterial community structure and its influencing factors in the shallow sea area of the Haizhou Bay, both surface and bottom water samples from six sites in the bay were selected and analyzed based on high-throughput sequencing technology. The results showed that the abun-dance and diversity of bacterial communities in the bottom water samples were better than those in the sur-face water samples. There was difference in the evolutionary direction of bacterial communities between the bottom and surface water samples, but the difference was not significant between the two bacterial communi-ties. A total of 2 491 operational taxonomic units (OTUs) were detected in the Haizhou Bay, belonging to 32 phyla, 74 classes, 116 orders, 200 families and 364 genera. At the phylum level, the dominant bacteria in the bay were Proteobacteria, Bacteroidetes and Cyanobacteria. Ammonium (NH4-N) and chlorophyll (Chl) were found to be the main environmental factors affecting bacterial community structure. The study demonstrated that the diversity and distribution of bacterial community structure in the shallow sea area of the Haizhou Bay had a certain correlation with environmental factors, which provided a theoretical basis for the sustaina-ble development of the shallow sea ecosystem in this area.

关键词

海州湾 / 高通量测序 / 细菌多样性 / 环境因子

Key words

Haizhou Bay / high-throughput sequencing / bacterial diversity / environmental factor