基于三维培养模型的辐射诱导表观遗传改变研究进展

Research Progress of Radiation-induced Epigenetic Changes Based on Three-dimensional Culture Model

摘要

相比于传统的单层细胞培养, 三维细胞培养(three-dimensional cell culture, TDCC)可以更准确地模拟体内细胞的生长环境, 近年来被越来越多地应用于生物基础研究。表观遗传修饰与细胞癌变等许多细胞过程联系紧密。辐射是肿瘤放射治疗的主要手段之一, 肿瘤放射治疗过程常常伴随着细胞表观遗传的动态变化, 它会诱导机体产生包括DNA甲基化、染色质重塑、组蛋白修饰和非编码RNA调控在内的表观遗传的改变。基于TDCC模型探究辐射诱导表观遗传调控发生的机制, 对于肿瘤患者的治疗和防护具有重要意义。

Abstract

Compared with the traditional monolayer culture, three-dimensional cell culture (TDCC) can more accurately simulate the growth environment of cells in vivo. In recent years, TDCC has been increasingly used in basic biological research. Epigenetic modification is closely linked to many cellular processes such as cell canceration. Radiation is one of the main means of tumor radiotherapy, the process of which is often accompanied by dynamic epigenetic changes, leading to DNA methylation, chromatin remodeling, histone modification and regulation of non-coding RNA. Exploring the mechanism of radiation-induced epigenetic regulation based on TDCC model is of great significance for treatment and protection of cancer patients.

关键词

三维细胞培养(TDCC) / 表观遗传调控 / 电离辐射 / 微RNA / DNA甲基化 / 染色质重塑

Key words

three-dimensional cell culture (TDCC) / epigenetic regulation / ionizing radiation / microRNA / DNA methylation / chromatin remodeling