多数据库联合探究TEKT2在卵巢癌中的作用

袁美莉; 李妍; 赵亮

生命科学研究 ›› 2023, Vol. 27 ›› Issue (2) : 170 -178.

生物信息学

多数据库联合探究TEKT2在卵巢癌中的作用

袁美莉, 李妍, 赵亮

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Exploration of the Role of TEKT2 in Ovarian Cancer by Combination of Multiple databases

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摘要

为了探讨筑丝蛋白2 (tektin 2, TEKT2)在卵巢癌(ovarian cancer, OV)中的表达情况、临床意义及功能机制, 为OV的早期诊断和治疗提供新思路, 本研究通过多个数据库的联合运用探讨TEKT2在OV中的作用。首先, 应用GEPIA2数据库分析TEKT2在OV中的表达情况, 同时使用GEPIA2数据库、TIMER数据库和Kaplan-Meier绘图平台探究TEKT2在OV中的预后价值; 其次, 采用Pearson分析探究TEKT2与肿瘤蛋白53 (tumor protein 53, TP53)在OV中的相关性, 并且基于Kaplan-Meier绘图平台探究TEKT2预后价值与TP53突变类型的关系; 再次, 通过TIMER数据库分析TEKT2与免疫细胞的相关性; 最后, 实时聚合酶链反应(real-time PCR)被用来验证TEKT2在OV细胞中的表达, 细胞增殖毒性检测(CCK-8)被用来评估 TEKT2与OV细胞增殖的关系。结果显示: TEKT2在OV中高表达, 与OV患者的预后较好相关, 且是一个独立预后因素; 在OV患者中, TEKT2与TP53的表达呈正相关, 并且在野生型TP53患者中, TEKT2具有预后价值; TEKT2的高表达与巨噬细胞和中性粒细胞密切相关, 其中TEKT2的臂水平缺失与中性粒细胞的浸润显著相关。进一步的CCK-8实验结果表明, TEKT2可抑制OV细胞的增殖。上述研究提示, TEKT2可能通过调节免疫细胞的浸润和抑制OV细胞的增殖影响OV患者预后。

Abstract

To investigate the expression, clinical significance and functional mechanism of tektin 2 (TEKT2) in ovarian cancer (OV), and provide new ideas in early diagnosis and treatment of OV, multiple databases were combined to explore the functions of TEKT2 in OV. Firstly, the expression of TEKT2 in the OV tissue was analyzed using GEPIA2 database. The prognostic value of TEKT2 in patients with OV was explored by GEPIA2 and TIMER databases and Kaplan-Meier plotter. Secondly, the correlation between TEKT2 and tumor protein 53 (TP53) in OV was explored by Pearson correlation analysis, and the relation between TEKT2 prognostic value and TP53 mutation type was analyzed using Kaplan-Meier plotter. Thirdly, the correlation between TEKT2 and immune cells was analyzed by TIMER database. Finally, the expression of TEKT2 in OV cells was verified by real time-PCR, and the effect of TEKT2 on the proliferation of OV cells was evalua-ted using Cell Counting Kit-8 (CCK-8). The results showed that TEKT2 was highly expressed in OV tissue, which was related to a good prognosis of OV patients, and TEKT2 was an independent prognostic factor. In OV patients, TEKT2 was positively correlated with the expression of TP53, and in wild-type TP53 patients, TEKT2 had prognostic value. High expression of TEKT2 was closely related to macrophages and neutrophils, in which obvious correlation existed between arm-level deletion of TEKT2 and neutrophil infiltration. More importantly, CCK-8 assay showed that TEKT2 could inhibit the proliferation of OV cells. In conclusion, this study found that TEKT2 may affect the prognosis of OV patients by regulating immune cell infiltration and inhibiting OV cell proliferation.