新烟碱类杀虫剂与孤独症谱系障碍风险的关联性研究

Association between neonicotinoid insecticides and autism spectrum disorder risk

摘要

目的 探讨儿童新烟碱类农药(NEOs)暴露与孤独症谱系障碍(ASD)发生风险的关联性。方法 本研究采用病例对照设计,选取武汉儿童医院的30名ASD患儿以及28名正常儿童作为研究对象。采用液相色谱串联质谱法检测其血浆样本中NEOs及其代谢物浓度,使用多元logistic回归模型评估单一NEO暴露与ASD发病相关性,采用加权分位数和(WQS)模型分析NEOs混合暴露和ASD风险的关联性。结果 病例组中5羟基吡虫啉(5OHIMI), 去甲基噻虫嗪(NTHM),氟啶虫胺腈(SFX)浓度较对照组明显升高(t=6.964, 2.276, 2.182, P＜0.05),且多元logistic回归分析显示,5OHIMI和NTHM暴露与ASD发生风险呈正相关(OR=47.738, 3.263, P＜0.05)。WQS模型分析显示,每增加一个单位的NEOs混合暴露浓度,ASD风险增加7.83倍[OR(95%CI)=7.83(0.480~15.180)]。结论 本研究发现NEOs暴露与ASD风险存在显著相关性,但其对ASD产生影响的具体作用机制仍待进一步研究。

Abstract

Objective To analyze the association between childhood exposure to neonicotinoid pesticides (NEOs) and the risk of autism spectrum disorder (ASD). Methods This case-control study enrolled 30 children with ASD and 28 typically developed children from Wuhan Children′s Hospital.Serum concentrations of NEOs and their metabolites were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS).Multivariate logistic regression models were used to assess the association between individual NEO exposure and ASD risk, and the weighted quantile sum (WQS) regression model was employed to analyze the association between mixed NEO exposure and ASD risk. Results Concentrations of 5-hydroxy-imidacloprid (5-OH-IMI), desmethyl-thiamethoxam (NTHM), and sulfoxaflor (SFX) were significantly higher in the ASD group compared to controls(t=6.964, 2.276, 2.182, P＜0.05).Multivariate logistic regression analysis revealed that 5-OH-IMI and NTHM were significantly associated with increased ASD risk (OR=47.738, 3.263, P＜0.05).WQS analysis indicated that mixed NEO exposure was positively associated with ASD risk (OR=7.83, 95% CI: 0.48 - 15.18). Conclusions This study found a significant association between NEO exposure and increased ASD risk.However, the specific mechanisms underlying this association require further investigation.

关键词

孤独症谱系障碍 / 新烟碱类杀虫剂 / 暴露

Key words

autism spectrum disorder / neonicotinoid insecticides / exposure

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基金资助

湖北省中央引导地方科技发展专项基金(2019ZYYD051)