蓝光通过脑-肾轴对大鼠肾结石形成的影响研究

Effects of blue light through the brain-kidney axis of rats on the formation of kidney stones

摘要

目的 分析蓝光通过脑-肾轴对大鼠肾结石形成的影响。方法 将40只大鼠随机分为A、B、C、D 4组，每组10只。C、D组大鼠采用10 g/L乙二醇+20 g/L氯化铵+100 g/L葡萄糖酸钙（2 mL/只）进行灌胃，A、B组大鼠给予等体积量生理盐水灌胃。A、C组大鼠从灌胃后第2天开始每日进行2次蓝光照射（1 h/次）干预，B、D组采用日光灯照射（方法同上），持续4周。收集大鼠24 h尿液后处死并收集其血液及肾脏组织样本。检测大鼠血清抗利尿激素（ADH）、尿Ca²⁺和尿草酸（Oxa）水平，ELISA法检测大鼠肾脏组织丙二醛（MDA）、超氧化物歧化酶（SOD）水平。Von Kossa染色观察肾组织病变以及肾脏中钙盐结晶情况。结果 与A、B组相比，C、D组大鼠肾脏组织中钙盐结晶积分较高，血清ADH、尿Ca²⁺、尿Oxa及肾组织中MDA水平较高，而肾组织SOD水平较低（P＜0.05）；与D组相比，C组肾脏组织中钙盐结晶积分较高，血清ADH、尿Ca²⁺、尿Oxa及肾组织中MDA水平较高，而肾组织SOD水平较低（P＜0.05）。结论 蓝光可能通过脑-肾轴促进血清ADH分泌和肾脏组织氧化应激反应，进而促进大鼠肾结石的形成。

Abstract

Objective To analyze the effects of blue light on the formation of kidney stones. Methods A total of 40 rats were randomly divided into 4 groups: A, B, C, and D, with 10 rats in each group. Rats in groups C and D were administered with a mixture of 10 g/L ethylene glycol, 20 g/L ammonium chloride, and 100 g/L calcium gluconate via gavage (2 mL per mouse), while rats in groups A and B received an equal volume of physiological saline via gavage. From the second day after gavage, rats in groups A and C were subjected to twice-daily blue light irradiation (one hour per session) as an intervention, while rats in groups B and D were subjected to fluorescent lamp irradiation using the same method. After 4 weeks of intervention, the 24-hour urine samples were collected, and the rats were then euthanized for the collection of blood and kidney tissue samples. Serum levels of antidiuretic hormone (ADH), urinary Ca²⁺, and urinary oxalate (Oxa) were measured. Levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in kidney tissues were detected using ELISA. Von Kossa staining was performed to observe pathological changes in kidney tissues and the presence of calcium salt crystals in the kidneys. Results Compared with groups A and B, groups C and D showed higher accumulation of calcium salt crystals in renal tissues, as well as elevated levels of ADH, urinary Ca²⁺, urinary Oxa, and MDA in renal tissues; additionally, the SOD level in renal tissues was lower (P<0.05). Compared with group D, group C exhibited higher accumulation of calcium salt crystals in renal tissues, along with increased levels of ADH, urinary Ca²⁺, urinary Oxa, and MDA in renal tissues; conversely, the SOD level in renal tissues was lower (P<0.05). Conclusion Blue light may increase the formation of kidney stones in rats by promoting the secretion of ADH in serum and oxidative stress in kidney tissues through the brain-kidney axis.

关键词

蓝光 / 脑-肾轴 / 大鼠 / 肾结石 / 抗利尿激素 / 丙二醛 / 超氧化物歧化酶

Key words

blue light / brain-kidney axis / rats / kidney stones / antidiuretic hormone / malondialdehyde / superoxide dismutase

参考文献

[1] ZENG G, MAI Z, XIA S, et al. Prevalence of kidney stones in China: an ultrasonography based cross-sectional study[J]. BJU Int, 2017, 120(1): 109-116.

[2] SOROKINI I, PEARLE MS. Medical therapy for nephrolithiasis: state of the art[J]. Asian J Urol, 2018, 5(4): 243-255.

[3] WAGNER CA. Etiopathogenic factors of urolithiasis[J]. Arch Esp Urol, 2021, 74(1): 16-23.

[4] 方道成, 陈立新, 胡媛媛. 肥胖与肾结石的研究进展[J]. 现代泌尿外科杂志, 2021, 26(4): 356-359.

[5] 方道成, 胡媛媛. 钙和维生素D与肾结石形成关系的研究进展[J]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(6): 653-656.

[6] YANG T, RICHARDS EM, PEPINE CJ, et al. The gut microbiota and the brain-gut-kidney axis in hypertension and chronic kidney disease[J]. Nat Rev Nephrol, 2018, 14(7): 442-456.

[7] 达静静, 查艳. 慢性肾脏病营养代谢中的脑-肾对话[J]. 中国实用内科杂志, 2019, 39(11): 937-940.

[8] JOHNS EJ, KOPP UC, DIBONA GF. Neural control of renal function[J]. Compr Physiol, 2011, 1(2): 731-767.

[9] OSBORN JW, TYSHYNSKY R, VULCHANOVA L. Function of renal nerves in kidney physiology and pathophysiology[J]. Annu Rev Physiol, 2021, 83: 429-450.

[10] VANDEWALLE G, MAQUET P, DIJK DJ. Light as a modulator of cognitive brain function[J]. Trends Cogn Sci, 2009, 13(10): 429-438.

[11] ANTEMIER G, SAMOILOA C, CLICHICI SV. Blue light-ocular and systemic damaging effects: a narrative review[J]. Int J Mol Sci, 2023, 24(6): 5998.

[12] 方道成, 陈立新, 王勇, 等. N-乙酰半胱氨酸对纳米细菌致大鼠肾结石形成的影响[J]. 现代泌尿外科杂志, 2021, 26(7): 611-614.

[13] ARITON DM, JIMENEZ-BALADO J, MAISTERRA O, et al. Diabetes, albuminuria and the kidney-brain axis[J]. J Clin Med, 2021, 10(11): 2364.

[14] WEN S, WANG C, GONG M, et al. An overview of energy and metabolic regulation[J]. Sci China Life Sci, 2019, 62(6): 771-790.

[15] 达静静. Klotho/Vasopressin通过脑肾轴调节2型糖尿病肾病异位肾脏脂质沉积的机制研究[D]. 贵州大学, 2022.

[16] 朱立志, 李郭锦, 田鹤, 等. 大鼠缺血性急性肾损伤对海马CA1区的形态学影响[J]. 天津医药, 2014(2): 164-166.

[17] LIU CC, YAMAZAKI Y, HECKMAN MG, et al. Tau and apolipoprotein E modulate cerebrovascular tight junction integrity independent of cerebral amyloid angiopathy in Alzheimer's disease[J]. Alzheimers Dement, 2020, 16(10): 1372-1383.

[18] GHOSHAL S. Renal and electrolyte disorders and the nervous system[J]. Continuum (Minneap Minn), 2023, 29(3): 797-825.

[19] GRONFIER C. Circadian clock and non-visual functions: the role of light in humans[J]. Biol Aujourd'hui, 2014, 208(4): 261-267.

[20] MURE LS. Intrinsically photosensitive retinal ganglion cells of the human retina[J]. Front Neurol, 2021, 12: 636330.

[21] ZHANG Z, LIU WY, DIAO YP, et al. Superior colliculus GABAergic neurons are essential for acute dark induction of wakefulness in mice[J]. Curr Biol, 2019, 29(4): 637-644.

[22] KAVOURAS SA, SUH HG, VALLET M, et al. Urine osmolality predicts calcium-oxalate crystallization risk in patients with recurrent urolithiasis[J]. Urolithiasis, 2021, 49(5): 399-405.

基金资助

上海市松江区科技攻关项目(2023SJKJGG84)