云南德宏3个不同澳洲坚果品种成熟期果实品质变化与最佳采收期预测

Changes in fruit quality during the ripening period and prediction of the optimal harvest time for three Macadamia cultivars in Dehong, Yunnan

摘要

【目的】探究澳洲坚果品种在不同采收期的表观性状与果实品质变化规律，为生产实践中确定各品种最佳采收期提供科学依据与技术支撑。【方法】以HAES344、迪思1号和Own Choice 3个澳洲坚果品种为研究对象，于8-10月分4个时间点采样，观测果实内果皮颜色，同步测定出种率、出仁率、一级果仁率、水分含量等指标，以及脂肪、可溶性糖、蛋白质等营养组分含量，并采用相关性分析、主成分分析及隶属函数法对各品种不同采收期的果实品质进行综合评价，筛选最佳采收期。【结果】随着采收期的延后，3个品种果实内果皮颜色均逐渐加深、光泽度提升；出仁率、一级果仁率随采收期延后呈显著上升趋势，迪思1号出仁率在采收末期达到峰值，为41.86%，Own Choice在采收后期及末期的一级果仁率稳定在99%以上；出种率在整个采收期内表现较为稳定。在营养组成上，3个品种果实脂肪含量（ w，后同）为67.90%~77.82%，变异系数均小于6%，且脂肪含量随采收期延后持续升高，与可溶性糖和蛋白质含量均呈负相关。【结论】HAES344与Own Choice最佳采收期为9月中旬，迪思1号为10月上旬。

Abstract

【Objective】As an important woody oil crop, macadamia relies heavily on harvest timing for optimal fruit quality. Currently, Yunnan, being China's largest macadamia growing region (accounting for 80% of the national planting area), lacks a scientifically based harvesting decision system, which severely limits the high-quality development of the industry. This study systematically investigated the dynamic changes in the appearance traits and fruit quality of the main macadamia ( Macadamia spp.) varieties in Dehong Autonomous Prefecture, Yunnan province, across different harvesting periods in order to provide solution to the problem of unstable fruit quality due to improper harvesting time, thereby establishing scientific harvesting standards. This, in turn, will enhance the commercial value and processing quality of macadamia fruits, thereby contributing to the sustainable development of Yunnan’s macadamia industry. 【Methods】Three main cultivars: HAES344, Disi No.1, and Own Choice were selected as test materials for this study. The study was conducted at the Manhuan plantation in Yingjiang County, Dehong Autonomous Prefecture, characterized by a South Asian tropical monsoon climate. During the fruit maturation period (August 1 to October 6), four equally spaced sampling time points were set. Nine healthy 13-year-old trees per variety, totaling 27 sample trees, were used. Fruit cluster samples were collected from four directions in the outer canopy. Appearance indicators such as endocarp color and glossiness were recorded, combining dissection methods with image recording. Fruit morphology parameters including fresh fruit mass, fresh nut mass, and dry mass were measured using an electronic balance with 0.01 g precision. Processing quality indicators such as shelling rate, kernel recovery, and first-grade kernel rate were calculated. Additionally, protein, starch and fat contents were determined. Differences among periods were compared using one-way ANOVA (LSD); Pearson correlation analysis was applied to reveal inter-indicator relationships, and key quality indicators were extracted through principal component analysis. Finally, a multi-dimensional comprehensive evaluation system was constructed using membership function analysis to establish a quantitative prediction model for the harvesting period. 【Results】Delaying harvest resulted in gradual endocarp darkening, increased glossiness, and enhanced lignification in all the three varieties. Disi No.1 performed best, displaying deep brown color with bright glossiness at final stage, indicating superior marketability. HAES344 colored slowly, showing uneven deep brown at maturity, while Own Choice maintained lighter pigmentation, reaching only light brown. For processing quality, kernel recovery and grade-one kernel rate significantly increased with delayed harvesting. The kernel recovery of HAES344 increased from 28.35% to 33.67%; that of Disi No.1 from 32.19% to 41.86%, and that of Own Choice from 33.36% to 36.04%. The kernel recovery rate of Disi No.1 peaked at 41.86% during the late harvest period. Own Choice maintained a stable grade-one kernel rate of over 99% in later harvesting stages. Shell-out rate remained stable (variation ≤3%). Nutritionally, fat content was generally high but varied among varieties. The fat content of the three cultivars ranged from 67.90% to 77.82%, with coefficients of variation all below 6%. HAES344 and Own Choice showed an initial increase followed by stabilization, while Disi No.1 first rose, then slowly declined, and finally stabilized. Soluble sugar and protein peaked early then decreased and stabilized. Disi No.1 had the highest soluble sugar content that reached 150 g·kg ^-1 soluble sugar, and HAES344 had the highest protein content of 82 g·kg ^-1. Moisture content consistently decreased to 18%-20%. Correlation analysis showed fat content positively correlated with kernel recovery and grade-one kernel rates, but negatively correlated with protein, moisture content, and shell-out rate. Principal component analysis indicated the first component (52.0% contribution) reflected fat content and processing quality (HAES344 highest). The second component (26.5% contribution) linked to soluble sugar and shell-out rate (Disi No.1 most prominent). Own Choice showed balanced performance. 【Conclusion】Based on membership function analysis, optimal harvesting periods differed significantly: HAES344 and Own Choice are best harvested in mid-September, while Disi No.1 should be harvested in early October. This finding fills a research gap for macadamia harvesting in Yunnan and provides a scientific basis for precise harvest scheduling. Future studies should incorporate meteorological factors to develop dynamic prediction models for optimizing harvest timing.

关键词

澳洲坚果 / 成熟期 / 内果皮颜色 / 果实品质 / 最佳采收期

Key words

Macadamia spp. / Maturity period / Endocarp color / Fruit quality / Optimal harvesting period

参考文献

[1] Zhang Shuyu, Wu Boxiao, Nie Yanli, Wang Yang, Kan Huan, Liu Yun. Analysis of flavor characteristics and unsaponifiable matter components in Yunnan-Produced Macadamia integrifolia oil [J/OL]. China Oils and Fats, 2025: 1-14. (2025-07-11)[ 2025-11-11]. https://doi.org/10.19902/j.cnki.zgyz.1003-7969.250291.

[2] Nagao M A, Hirae H H, Stephenson R A . Macadamia: Cultivation and physiology [J]. Critical Reviews in Plant Sciences, 1992, 10(5): 441-470.

[3] Zhang Tao, Song Haiyun, Tu Xinghao, He Peng, Chen Xi, Wei Zhejun, Zheng Shufang, Wang Wenlin. Stability and difference analysis of Macadamia nut oil characteristics among different production areas in Guangxi [J]. Journal of the Chinese Cereals and Oils Association, 2025, 40(5): 135-143.

[4] Duan Minxian, Zhang Birong, Yang Fan, Li Xuejia, Shi Wenbin, Yan Suyun, Tang Shaoping, Zhao Jun, Zhou Xianyan. Analysis of fatty acid compositions and concentrations in the kernel of seven main macadamia cultivars in Yunnan[J]. Journal of Fruit Science, 2025, 42(4): 828-839.

[5] Zhou Tao, Wang Dawei, Nie Yanli, Wang Zhengcheng. Phenotypic diversity analysis of macadamia germplasm resources in Yunnan[J]. Journal of Southern Agriculture, 2025, 56(3): 742-750.

[6] Peng Jingna, Qi Tingmei, Zhang Yuejiang, Fu Xiaoping, Lin Qi, Bao Yuanyuan. Effect of macadamia oil addition on the oxidative stability of walnut oil[J]. Journal of the Chinese Cereals and Oils Association, 2025, 40(4): 117-125.

[7] Pan Yinxixue, Wang Xuezhao, Li Xiaona, Zhang Lin, Wang Leiguang. The impacts of climate change on the potential ecologically suitable plantation area of Macadamia spp. in China [J/OL]. Guihaia, 2025: 1-18(2025-09-24)[ 2025-10-30]. https://link.cnki.net/urlid/45.1134.Q.20250923.1805.004.

[8] Lu Bin, Li Xiaolin, Gu Lishu, Hou Xianfeng, Li Yupin, Zheng Yi. The impact of climate change on the suitable growth zone of Macadamia integrifolia in Yunnan province [J]. Journal of Ecology and Rural Environment, 2025, 41(7): 866-873.

[9] Ning Ya, Chen Yuchun, Wu Zhonghua, He Shuangling, He Xiyong, Yang Tingmei, Yue Hai. Analysis of fungal community structure and diversity in rhizosphere soil of Macadamia in different regions of Yunnan province [J]. Southwest China Journal of Agricultural Sciences, 2025, 38(7): 1479-1488.

[10] Lu Jingyi, Li Ya, Shuai Xixiang, Zhang Ming, Wu Zhenxian, Du Liqing, Deng Xu. Effect of different storage temperatures on the quality and metabolic changes of fresh macadamia nuts[J]. Journal of Fruit Science, 2025: 1-18(2025-09-04)[ 2025-10-30]. https://doi.org/10.13925/j.cnki.gsxb.20250379.

[11] Zhang Cheng, Zhang Hong, Su Jianrong, Tu Xinghao, Xu Juan, Li Ying, Liu Lanxiang, Tang Xiaoning, Ma Jinju. Preservation effect of gallnut tannin on fresh macadamia nuts[J]. Food Science, 2025, 46(14): 311-320.

[12] Shuai X X, Dai T T, Chen M S, Liu C M, Ruan R, Liu Y H, Chen J . Characterization of lipid compositions, minor components and antioxidant capacities in macadamia (Macadamia integrifolia) oil from four major areas in China [J]. Food Bioscience, 2022, 50: 102009.

[13] Zhu Yi, Yang Hongxia, Zheng Weikang, Tang Shaoping, Chai Lijun, Yang Fan, Ma Zhaocheng. Analysis of agronomic traits and fatty acid composition of macadamia germplasm resources in Yunnan province[J]. Acta Horticulturae Sinica, 2025, 52(7): 1707-1717.

[14] Wan Jifeng, Zou Minghong, Chen Jing, Song Ximei, Yang Qian, Luo Lianfang, Zeng Hui. Study on numerical taxonomy of Macadamia spp. germplasm resources [J]. Journal of Fruit Science, 2022, 39(10): 1798-1812.

[15] Kang Z M, Cai H, Guo G Z, Zeng H, Wang W L, Tu X H . Physiological response of macadamia (Macadamia integrifolia) seedlings to drought stress [J]. Horticulturae, 2025, 11(4): 347.

[16] Huang Sijie, Huang Yunpeng, Li Bing, Ye Weiyan, Yang Xiangyan, Yang Weihai, Cai Yuanbao. Comprehensive evaluation of cold resistance of eight macadamia varieties under low temperature stress[J]. Guihaia, 2025, 45(9): 1724-1734.

[17] Shuai X X, Dai T T, Chen M S, Liang R H, Du L Q, Chen J, Liu C M . Comparative study on the extraction of macadamia (Macadamia integrifolia) oil using different processing methods [J]. LWT, 2022, 154: 112614.

[18] Aruwajoye N N, Buthelezi N M D, Mditshwa A, Tesfay S Z, Magwaza L S . Assessing the impact of roasting temperatures on biochemical and sensory quality of macadamia nuts (Macadamia integrifolia) [J]. Foods, 2023, 12(11): 2116.

[19] He Peng, Zhang Tao, Song Haiyun, Zheng Shufang, Qin Zhenshi, Huang Xiyun, Tang Xiuhua, Wang Wenlin. Determination of harvesting time of Gui Re No. 1 macadamia nuts suitable for processing of slotted nut[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(8): 1825-1831.

[20] He Peng, Zhang Tao, Song Haiyun, Zheng Shufang, Qin Zhenshi, Tan Qiujin, Huang Xiyun, Tang Xiuhua, Xu Peng, Chen Haisheng, Wang Wenlin. Determination of suitable harvesting time of Macadamia integrifolia cv. HAES695 for processing of slotted nut [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1489-1496.

[21] Wan Jifeng, Lu Zhiqiang, Zou Minghong, Chen Jing, Song Ximei, Yang Qian, Dong Lihong, Luo Lianfang, Zeng Hui. Changes in endocarp color, kernel quality and prediction of optimal harvest time during the ripening period of Macadamia integrifolia [J]. South China Fruits, 2023, 52(6): 72-75.

[22] Ma Jing, Gong Lidan, Yue Hai, Li Zhiqiang, Wu Chao, Chen Yuchun, Ning Ya, Guo Gangjun, He Xiyong. Fruit development and quality change of ‘O. C’ cultivar in macadamia[J]. Journal of West China Forestry Science, 2024, 53(4): 76-84.

[23] Tan Qiujin, Chen Haisheng, Wei Yuanrong, Xu Peng, Zheng Shufang, Huang Xiyun, He Xianyang, Qin Zhenshi, Wang Wenlin. Nutritional analysis and evaluation of kernel of Macadamia integrifolia [J]. Journal of the Chinese Cereals and Oils Association, 2021, 36(2): 150-154.

[24] Tan Qiujin, Wei Yuanrong, Huang Xiyun, Zhang Tao, Xu Peng, Song Haiyun, Wang Wenlin, Zheng Shufang. Analysis of fruit characteristics and nutrients of 10 accessions of Macadamia integrifolia [J]. Journal of Fruit Science, 2021, 38(5): 672-680.

[25] Lu Chaozhong, Zeng Hui, Zhang Hanzhou. Studies on yield, kernel quality and wind resistance of macadamia cultivar own choice[J]. Journal of Fruit Science, 2004, 21(1): 82-84.

[26] Li Xingze, Wei Xinglan, Zeng Huiping, Dong Qiong. Effect of foliar fertilization with variable ration on seeding growth and non-structural carbohydrates of Cyphomandra betacea [J]. Southwest China Journal of Agricultural Sciences, 2024, 37(12): 2765-2774.

[27] Zeng Huiping, Sun Lijuan, Cha Xiaofei, Guo Huanxian, Li Xingze, Dong Qiong. Effects of precipitation and precipitation interval on growth and photosynthetic fluorescence characteristics of Cyphomandra betacea seedlings [J]. Journal of Plant Resources and Environment, 2025, 34(5): 78-88.

[28] Li Yang, Geng Jianjian, Ma Jing, Wu Chao, Li Zhiqiang, Wang Kang, Gong Lidan, He Xiyong, Tao Liang. Introduction performance of 16 macadamia varieties in Yingjiang of Yunnan province[J]. Non-wood Forest Research, 2024, 42(3): 131-139.

[29] Song Ximei, Zou Minghong, Kang Zhuanmiao, Luo Lianfang, Du Liqing, Wan Jifeng, Yang Qian, Wang Zhao, Zeng Hui. A new cultivar macadamia ‘Nanya 116’ of high kernel rate[J]. Acta Horticulturae Sinica, 2025, 52(S1): 67-68.

[30] Lai Hairong, Guo Sujuan. Observation on the anatomical structure of chestnut nut pericarp and seed coat development[J]. Journal of Northwest A & F University (Natural Science Edition), 2025, 53(9): 41-53.

[31] Luo Yiran, Shen Dezhou. Main traits and pest control technologies of Macadamia integrifolia in Mangshi, Yunnan [J]. Practical Technology and Information for Fruits, 2022(4): 42-44.

[32] Ye Tiantian, Liu Xue, Liang Xiaojie, Su Shuchai. Comparison of fruit quality differences between two varieties of Camellia oleifera fruit at different maturity [J]. China Oils and Fats, 2023, 48(6): 85-90.

[33] Lu Hongli, Aliyas Ahmat, Gong Xiang, Zhang Rui, Mutalip Aiheti. Dynamic study on fruit quality changes of “Wen 185” walnut at different harvest periods[J]. Xinjiang Farm Research of Science and Technology, 2022, 45(2): 24-28.

[34] Kang Zhuanmiao, He Fengping, Geng Jianjian, Zhu Wenhua, Lei Chaoyun, Liu Fanzhi, Zhang Yan, Wang Daigu, Li Xiangyong, Fan Jianxin, Lu Jiaju, Zeng Hui, Tao Liang. Evaluation of quality characters of macadamia suitable for planting in Guizhou[J]. Non-wood Forest Research, 2020, 38(4): 117-124.

[35] Ma Shangxuan, Hu Xiaojing, Fu Jiarong, Wei Yuanmiao, Xu Wenting, Jiang Jiatai, Wang Jiatang, Li Hong, Guo Gangjun, He Xiyong. Quality characteristics and comprehensive evaluation of different varieties of macadamia nuts in Xishuangbanna of Yunnan[J]. South China Fruits, 2024, 53(6): 68-75.

[36] Liu Fenglan, Han Hongwei, Mao Jinmei, Wang Qin, Li Yong, Wang Jianyou. Comparison of crude fat contents and oil fatty acid compositions of different species of almond kernel in different harvest time[J]. China Oils and Fats, 2018, 43(6): 104-107.

[37] Tan Yali, Gao Mengxiang, Li Xiaojie, Zhou Yingjie, Xiong Jian, Zeng Ziqi, Li Xiao, Yang Hua. Quality of mainly cultivated walnuts in Hubei province of China at different harvest periods[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1459-1468.

[38] Wang Yifeng, Gong Zhengrong, Hu Wenbin, Liu Yihong, Wang Mingxia, Chen Yaonian, Zhao Shuling. Effect of harvest time on browning of walnut endocarps during storage[J]. Non-wood Forest Research, 2024, 42(3): 255-263.

[39] Wei Qin, Chang Yuemei, Gao Junxian, Li Yongchen, Hao Yanyan. Study on harvest period of fresh walnut ‘Lipin No. 2’[J]. Acta Agriculturae Universitatis Jiangxiensis, 2024, 46(5): 1172-1185.

[40] Zhao Menghuan, Xu Junyuan, Liu Yali, Li Pengcheng, Liu Zhen, Li Zhi, Geng Xiaodong, Dai Li, Liu Yi, Wang Yanmei. Analysis of fruit quality of Idesia polycarpa Maxim at different harvest periods [J]. Journal of Henan Agricultural University, 2024, 58(1): 69-77.

[41] Chen Zixi, Chen Yaobing, Guo Xiaoni, Liu Hanzhen, Huang Xiufang, Luo Kai. Changes of aroma components in fruit and oil of Idesia polycarpa in different harvest periods [J]. Journal of the Chinese Cereals and Oils Association, 2023, 38(5): 108-114.

[42] Zou Yuting, Zhu Mingwei, Li Yongrong, Zhai Jinting, Li Shuxian. Dynamic changes in nutrients content and related enzymes activity during Paeonia ostii ‘Feng Dan’ seeds development [J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2021, 45(5): 62-70.

[43] Su Jing, Zhu Lingcheng, Liu Xi, Peng Yunjing, Ma Baiquan, Ma Fengwang, Li Mingjun. Research progress on sugar metabolism and concentration regulation in fruit[J]. Journal of Fruit Science, 2022, 39(2): 266-279.

[44] Zhou Changfu, Yao Xiaohua, Lin Ping, Lu Jing. Growth characteristics and dynamic analysis of water and oil content on oil-tea camellia fruit[J]. Journal of Yangzhou University (Agricultural and Life Science Edition), 2013, 34(3): 49-53.

[45] Wang Yaping, Fei Xueqian, Shi Xiaoli, Ye Xiaofei, Guo Shaohai, Luo Fan. Effects of harvest time and treatment methods on nutrient accumulation of oil-tea camellia seed and its oil[J]. China Oils and Fats, 2017, 42(4): 20-23.

[46] Tan Qiujin, Wang Wenlin, Wei Yuanrong, Zheng Shufang, Huang Xiyun, He Xianyang, Chen Haisheng. Diversity analysis of fruit traits related to yield in Macadamia germplasms [J]. Journal of Fruit Science, 2019, 36(12): 1630-1637.

[47] Ren Erfang, Liu Gongde, Ai Jingwen, Cheng Sanhong, Luo Chaodan, Luo Xiaojie. Effects of different drying methods on macadamia nut quality[J]. Food Research and Development, 2021, 42(12): 136-141.

[48] Tan Qiujin, Wang Zhengcheng, Yang Xianwu, Shi Yuanjuan, Kang Zhuanmiao, Wang Wenlin. A new Macadamia cultivar ‘Zhengcheng 1’ [J]. Acta Horticulturae Sinica, 2024, 51(S2): 53-54.

基金资助

云南省科技厅重大科技专项计划(202402AE090029)

中央财政林业科技推广示范(云﹝2025﹞TG21-1号)

云南省科技厅乡村振兴科技专项(202304BI090032-56)

中央财政林业科技推广示范(云﹝2023﹞TG09号)