Abstract:【Objective】The collection, evaluation, and rational utilization of sea buckthorn germplasm resources form the foundation for breeding superior varieties and developing effective production practices. Qinghai Province possesses an abundance of sea buckthorn germplasm resources. This study aimed to investigate the genetic diversity of fruit and seed phenotypic traits among local and introduced sea buckthorn varieties in alpine region of Qinghai, with the objective of identifying germplasm exhibiting exceptional seed and fruit characteristics.【Methods】This study systematically evaluated the phenotypic traits of fruits and seeds from 36 sea buckthorn germplasm samples collected in alpine region of Qinghai. The aim was to establish a reference for assessing fruit quality, improving germplasm breeding, and supporting the sustainable development of the sea buckthorn industry in Qinghai Province. Evaluated quality traits included fruit shape, seed shape, seed coat color, and grooves, while quantitative traits encompassed berry stalk length, berry longitudinal and transverse diameters, berry shape index, berry volume, single berry weight, seed length and width, seed shape index, seed volume, 1000-grain weight, exocarp and endocarp mass, as well as exocarp and endocarp thickness. Subsequent analyses included diversity, correlation, and cluster analyses of these phenotypic traits, culminating in a comprehensive evaluation through principal component analysis.【Results】The fruits and seeds of 36 sea buckthorn germplasms exhibited significant genetic diversity, with quality traits showing a diversity index ranging from 0 to 1.14. Fruit shapes displayed the highest variation types and the largest diversity index, while seed grooves exhibited the smallest diversity index, present in all germplasm seeds. It indicated that the distribution of this trait was relatively stable among different description types. Among the 36 sea buckthorn germplasms, oblate ones were the most common, accounting for 40.74%, followed by round ones, accounting for 38.43%, and cylinder ones had the lowest proportion, only 2.78%. The coefficient of variation for the 15 quantitative traits varied from 11.46% to 68.42%. Notably, exocarp thickness and endocarp mass demonstrated substantial variation, with coefficients of variation exceeding 60%, and berry stalk length, berry volume, single berry weight, seed volume, exocarp mass, and endocarp thickness, were all above 30%, indicating considerable dispersion of phenotypic traits among different sea buckthorn germplasms. The diversity index for quantitative traits ranged from 1.25 to 2.08. Berry stalk length, berry transverse diameter, single fruit weight, seed length and width, seed shape index, seed volume, 1000-grain weight, and endocarp thickness all exhibited diversity indices exceeding 2.00, classifying them as highly variable traits. The genetic diversity index for the remaining traits fell between 1.00 and 2.00. The correlation analysis revealed intricate and strong correlations among diverse phenotypic traits in different sea buckthorn germplasms. Subsequent cluster analysis categorized 36 sea buckthorn germplasm resources into three distinct groups: the first group featured medium-sized fruits with thin and light fruit skins; the second group exhibited small fruits with thick fruit skins; the third group showcased large fruits with elongated fruit stalks. The principal component analysis demonstrated that the cumulative contribution rate of the three principal components identified was 80.011%. Specifically, the first principal component primarily reflected the quality and shape of fruits and seeds, the second principal component predominantly characterized the exocarp thickness, and the third principal component was mainly associated with the endocarp thickness. The comprehensive assessment indicated that the highest overall score was recorded in 2013-05 (5.510).【Conclusion】The variation in fruit and seed quality traits among sea buckthorn germplasms in alpine region of Qinghai is relatively extensive, and the diversity index for quantitative traits is notably high. Through a combination of correlation analysis and principal component analysis, five key indicators for assessing sea buckthorn plant traits are identified: berry longitudinal diameter, seed length, single fruit weight, 1000-grain weight, and berry peel thickness. These indicators can serve as crucial reference points for subsequent quality analysis, evaluation of sea buckthorn germplasm resources, and variety breeding efforts. The comprehensive scores of fruit and seed phenotypic traits of six sea buckthorn germplasms namely 2013-05, Zayou No. 2, Fuza, LJ-10-03, LJ-10-56, and Zayou No. 1, rank among the highest as a result of integrating various evaluation methods, and are suitable for breeding sea buckthorn varieties and for the rational development and utilization in alpine region of Qinghai.
| [1] |
夏立泉， 韩富忠， 冶俊森 . 沙棘在吉林西部退化土地治理中的作用[J]. 国际沙棘研究与开发， 2009， 7(2)： 20-23.
|
| [2] |
Xia Liquan， Han Fuzhong， Ye Junsen. Significance of seabuckthorn in degraded land management of west Jilin Province[J]. The Global Seabuckthorn Research and Development， 2009， 7(2)： 20-23.
|
| [3] |
周帅 . 西藏 3 种沙棘植物形态解剖特征及其环境适应性研究[D]. 林芝： 西藏农牧学院， 2023.
|
| [4] |
Zhou Shuai. Morphological and anatomical characteristics and environmental adaptability of three species of Hippophae in Tibet [D]. Linzhi： Xizang Agricultural and Animal Husbandry University， 2023.
|
| [5] |
Liu J W， Bai J W， Shao C T， Yao S N， Xu R， Duan S Y， Wang L B， Xu Y Q， Yang Y . Optimization of ultrasound-assisted aqueous two-phase extraction of polysaccharides from seabuckthorn fruits using response methodology，physicochemical characterization and bioactivities[J]. Journal of the Science of Food and Agriculture， 2023， 103(6)： 3168-3183.
|
| [6] |
Hao Y K， Zhou F B， Dong J J， Wang Y， Lang Z F， Li S， Li S . Study on the role of flavonoids derived extract from seed residues of Hippophae rhamnoides on high-fat diet induced obese mice [J]. Journal of King Saud University - Science， 2020， 32(2)： 1597-1603.
|
| [7] |
李曦光， 李萧婷， 王蕾， 高健， 罗磊 . 基于空间差异的新疆沙棘资源果实品质及营养成分分析[J]. 农业工程学报， 2023， 39(23)： 268-275.
|
| [8] |
Li Xiguang， Li Xiaoting， Wang Lei， Gao Jian， Luo Lei. Fruit quality and medicinal components in Xinjiang sea buckthorn under spatial distribution differences[J]. Transactions of the Chinese Society of Agricultural Engineering， 2023， 39(23)： 268-275.
|
| [9] |
方贵平， 毕金峰， 刘春海， 岳丽华， 李旋， 刘佳， 王雅 . 中国四个地区代表性沙棘果实综合品质评价[J]. 农业工程学报， 2022， 38(21)： 249-260.
|
| [10] |
Fang Guiping， Bi Jinfeng， Liu Chunhai， Yue Lihua， Li Xuan， Liu Jia， Wang Ya. Evaluation of the comprehensive quality of sea buckthorn fruit in four areas of China[J]. Transactions of the Chinese Society of Agricultural Engineering， 2022， 38(21)： 249-260.
|
| [11] |
Wang K W， Xu Z Z， Liao X J . Bioactive compounds，health benefits and functional food products of sea buckthorn：A review[J]. Critical Reviews in Food Science and Nutrition， 2022， 62(24)： 6761-6782.
|
| [12] |
刁松锋， 邵文豪， 姜景民， 董汝湘， 孙洪刚 . 基于种实性状的无患子天然群体表型多样性研究[J]. 生态学报， 2014， 34(6)： 1451-1460.
|
| [13] |
Diao Songfeng， Shao Wenhao， Jiang Jingmin， Dong Ruxiang， Sun Honggang. Phenotypic diversity in natural populations of Sapindus mukorossi based on fruit and seed traits [J]. Acta Ecologica Sinica， 2014， 34(6)： 1451-1460.
|
| [14] |
刘志龙， 虞木奎， 唐罗忠， 方升佐 . 不同种源麻栎种子形态特征和营养成分含量的差异及聚类分析[J]. 植物资源与环境学报， 2009， 18(1)： 36-41.
|
| [15] |
Liu Zhilong， Yu Mukui， Tang Luozhong， Fang Shengzuo. Variation and cluster analyses of morphological characters and nutrient content of Quercus acutissima seed from different provenances [J]. Journal of Plant Resources and Environment， 2009， 18(1)： 36-41.
|
| [16] |
赵鑫丹， 张东为， 戈素芬， 李前， 阮成江 . 沙棘果实和种子表型性状及变异分析[J]. 种子， 2023， 42(9)： 105-112.
|
| [17] |
Zhao Xindan， Zhang Dongwei， Ge Sufen， Li Qian， Ruan Chengjiang. Analysis on variation and phenotypic characters in berries and seeds of Hippophae rhamnoides [J]. Seed， 2023， 42(9)： 105-112.
|
| [18] |
余天蓝， 程功， 曼苏尔·那斯尔， 赵世荣， 牛莹莹， 赵英， 廖康 . 新疆 6 个天然居群野生沙棘果实性状多样性研究[J]. 果树学报， 2018， 35(5)： 548-556.
|
| [19] |
Yu Tianlan， Cheng Gong， Mansur Nasir， Zhao Shirong， Niu Yingying， Zhao Ying， Liao Kang. Study on fruit diversity of wild seabuckthorn in Xinjiang[J]. Journal of Fruit Science， 2018， 35(5)： 548-556.
|
| [20] |
高文于， 杨际学， 吕庆鑫， 白有志， 杨红 . 西藏 5 种野生沙棘果实及种子表型性状差异分析[J]. 经济林研究， 2025， 43(2)： 148-157.
|
| [21] |
Gao Wenyu， Yang Jixue， Lü Qingxin， Bai Youzhi， Yang Hong. Analysis of phenotypic differences in fruits and seeds of 5 wild Hippophae rhamnoides species in Xizang [J]. Non-wood Forest Research， 2025， 43(2)： 148-157.
|
| [22] |
戈素芬， 张东为， 任丽华， 胡建忠， 温秀凤 . 辽西 8 个俄罗斯第三代大果沙棘良种的果实特性和生长节律的比较[J]. 经济林研究， 2021， 39(3)： 49-57.
|
| [23] |
Ge Sufen， Zhang Dongwei， Ren Lihua， Hu Jianzhong， Wen Xiufeng. Comparison of berry characteristics and growth rhythm of 8 third generation seabuckthorns from Russia in western Liaoning，China[J]. Non-wood Forest Research， 2021， 39(3)： 49-57.
|
| [24] |
谢嗣荣， 吴帅， 蔡金峰， 曹福亮 . 内蒙古不同种群沙棘种子性状变异及其稳定性分析[J]. 林业科技开发， 2015， 29(6)： 55-58.
|
| [25] |
Xie Sirong， Wu Shuai， Cai Jinfeng， Cao Fuliang. The variation and stability of seed characters among different places of origin of the Hippophae rhamnoides in Inner Mongolia [J]. China Forestry Science and Technology， 2015， 29(6)： 55-58.
|
| [26] |
张学峰， 杨双云， 蔡红， 黄豆， 叶代全， 边黎明. 遗传改良对杉木针叶和种实性状的影响[J]. 广西植物， 2023， 43(7)： 1296-1307.
|
| [27] |
Zhang Xuefeng， Yang Shuangyun， Cai Hong， Huang Dou， Ye Daiquan， Bian Liming. Effects of genetic improvement on traits of needle and cone of Cunninghamia lanceolata [J]. Guihaia， 2023， 43(7)： 1296-1307.
|
| [28] |
赵崇斌， 郭乙含， 李舒庆， 徐红霞， 黄天启， 林顺权， 陈俊伟， 杨向晖 . 宁海白×大房枇杷 F1 杂交群体果实性状的相关性及遗传分析[J]. 果树学报， 2021， 38(7)： 1055-1065.
|
| [29] |
Zhao Chongbin， Guo Yihan， Li Shuqing， Xu Hongxia， Huang Tianqi， Lin Shunquan， Chen Junwei， Yang Xianghui. Correlation and genetic analysis of fruit traits in F1 hybrid population of loquat generated from Ninghaibai × Dafang[J]. Journal of Fruit Science， 2021， 38(7)： 1055-1065.
|
| [30] |
Zhang Z R， Li W Y， Dong Y Y， Liu J X， Lan Q Y， Yang X， Xin P Y， Gao J . Geographic cline and genetic introgression effects on seed morphology variation and germination fitness in two closely related pine species in Southeast Asia[J]. Forests， 2022， 13(3)： 374.
|
| [31] |
Li Z X， Wang W H， Zhang H X， Liu J H， Shi B Y， Dai W Z， Liu K W， Zhang H G . Diversity in fruit morphology and nutritional composition of Juglans mandshurica Maxim in Northeast China [J]. Frontiers in Plant Science， 2022， 13： 820457.
|
| [32] |
吴琼 . 中国沙棘、云南沙棘表型多样性研究[D]. 兰州： 西北师范大学， 2007.
|
| [33] |
Wu Qiong. Study on phenotypic diversity in H. rhamnoides subsp. sinensis and H. rhamnoides subsp. yunnanensis with a discussion on origin of H. goniocarpa [D]. Lanzhou： Northwest Normal University， 2007.
|
| [34] |
张建国， 罗红梅， 黄铨， 单金友， 王春艳 . 大果沙棘不同品种果实特性比较研究[J]. 林业科学研究， 2005， 18(6)： 643-650.
|
| [35] |
Zhang Jianguo， Luo Hongmei， Huang Quan， Shan Jinyou， Wang Chunyan. A comparative study on berry characteristics of large berry cultivars of sea buckthorn[J]. Forest Research， 2005， 18(6)： 643-650.
|
| [36] |
赵东晓 . 我国第二阶段第二期（2021-2030 年）沙棘育种工作草案[J]. 中国水土保持， 2020(2)： 32-33.
|
| [37] |
Zhao Dongxiao. The draft of seabuckthorn breeding working in phase ⅱ of the second stage of China from 2021 to 2030[J]. Soil and Water Conservation in China， 2020(2)： 32-33.
|
| [38] |
王丹， 陈超 . 砒砂岩区沙棘生态工程建设的实践与思考[J]. 中国水土保持， 2022(9)： 51-54.
|
| [39] |
Wang Dan， Chen Chao. Practice and consideration on ecological engineering construction of seabuckthorn in arsenic sandstone areas[J]. Soil and Water Conservation in China， 2022(9)： 51-54.
|
| [40] |
唐克， 吴雨蹊， 王蕊， 陶猛， 单金友， 周双， 焦奎宝， 王明洁， 丁健. 东北黑土区沙棘杂交品种（系）果实性状及营养成分比较[J]. 经济林研究， 2023， 41(3)： 286-295.
|
| [41] |
Tang Ke， Wu Yuxi， Wang Rui， Tao Meng， Shan Jinyou， Zhou Shuang， Jiao Kuibao， Wang Mingjie， Ding Jian. Comparison of fruit characters and nutrient components of seabuckthorn hybrids (lines) in the black soil region of Northeast China[J]. Non-wood Forest Research， 2023， 41(3)： 286-295.
|
| [42] |
宋洪伟， 张冰冰 . 沙棘种质资源描述规范和数据标准[M]. 北京： 中国农业出版社， 2006.
|
| [43] |
Song Hongwei， Zhang Bingbing. Descriptors and data standard for seabuckthorn (Hippophae rhamnoides L.) [M]. Beijing： China Agriculture Press， 2006.
|
| [44] |
梅源， 金小朵， 段冰冰， 王新， 苏周晨星， 任一曌， 刘旭 . 赤霞珠葡萄果实酚成熟度与果皮质地特性的关系[J]. 西北林学院学报， 2021， 36(1)： 132-138.
|
| [45] |
Mei Yuan， Jin Xiaoduo， Duan Bingbing， Wang Xin， Su Zhouchenxing， Ren Yizhao， Liu Xu. Relationship between phenolic maturity and skin textural properties for Cabernet Sauvignon (Vitis vinifera) grapes [J]. Journal of Northwest Forestry University， 2021， 36(1)： 132-138.
|
| [46] |
张建霞， 姜成英， 吴文俊， 戚建莉， 金高明， 张荣， 姚玉芳 . 陇南 98 份油橄榄种质表型多样性分析[J]. 果树学报， 2025， 42(9)： 1991-2002.
|
| [47] |
Zhang Jianxia， Jiang Chengying， Wu Wenjun， Qi Jianli， Jin Gaoming， Zhang Rong， Yao Yufang. Phenotypic diversity analysis of 98 olive germplasm accessions in Longnan[J]. Journal of Fruit Science， 2025， 42(9)： 1991-2002.
|
| [48] |
李颖， 张树航， 郭燕， 张馨方， 王广鹏 . 中国板栗可溶性糖相关性状多样性分析[J]. 植物遗传资源学报， 2023， 24(2)： 493-504.
|
| [49] |
Li Ying， Zhang Shuhang， Guo Yan， Zhang Xinfang， Wang Guangpeng. Diversity analysis of soluble sugar related traits in Chinese chestnut[J]. Journal of Plant Genetic Resources， 2023， 24(2)： 493-504.
|
| [50] |
段九菊， 梁峥， 贾民隆， 宋卓琴， 张超， 曹冬梅 . 山西省萱草属野生资源的表型多样性分析[J]. 植物资源与环境学报， 2021， 30(6)： 29-38.
|
| [51] |
Duan Jiuju， Liang Zheng， Jia Minlong， Song Zhuoqin， Zhang Chao， Cao Dongmei. Phenotypic diversity analysis of wild resources of Hemerocallis in Shanxi province [J]. Journal of Plant Resources and Environment， 2021， 30(6)： 29-38.
|
| [52] |
Liu D L， Wang X Q， Li W S， Li J J， Tan W B， Xing W . Genetic diversity analysis of the phenotypic traits of 215 sugar beet germplasm resources[J]. Sugar Tech， 2022， 24(6)： 1790-1800.
|
| [53] |
董胜君， 王若溪， 张皓凯， 陈建华， 刘立新， 于庆福 . 不同种源东北杏果实表型性状多样性分析[J]. 植物资源与环境学报， 2020， 29(6)： 42-50.
|
| [54] |
Dong Shengjun， Wang Ruoxi， Zhang Haokai， Chen Jianhua， Liu Lixin， Yu Qingfu. Analysis on diversity of fruit phenotypic characters of Armeniaca mandshurica from different provenances [J]. Journal of Plant Resources and Environment， 2020， 29(6)： 42-50.
|
| [55] |
尹明宇， 高福玲， 乌云塔娜 . 内蒙古西伯利亚杏种质资源表型多样性研究[J]. 植物遗传资源学报， 2017， 18(2)： 242-252.
|
| [56] |
Yin Mingyu， Gao Fuling， Wuyun Tana. High-level genetic diversity of Siberian Apricot (Armeniaca sibirica) in Inner Mongolia revealed by phenotyping [J]. Journal of Plant Genetic Resources， 2017， 18(2)： 242-252.
|
| [57] |
董胜君， 孙永强， 陈建华， 卢彩云， 刘权钢， 刘立新 . 野杏无性系表型性状多样性分析及综合评价[J]. 植物遗传资源学报， 2020， 21(5)： 1156-1166.
|
| [58] |
Dong Shengjun， Sun Yongqiang， Chen Jianhua， Lu Caiyun， Liu Quangang， Liu Lixin. Phenotypic traits diversity analysis and comprehensive evaluation of Armeniaca vulgaris var. ansu clones [J]. Journal of Plant Genetic Resources， 2020， 21(5)： 1156-1166.
|
| [59] |
董胜君， 陈建华， 刘立新， 刘明国， 吴月亮， 夏泽臻 . 不同种源西伯利亚杏表型性状多样性研究[J]. 沈阳农业大学学报， 2018， 49(6)： 686-696.
|
| [60] |
Dong Shengjun， Chen Jianhua， Liu Lixin， Liu Mingguo， Wu Yueliang， Xia Zezhen. Research on phenotypic trait diversity of Armeniaca sibirica from different provenances [J]. Journal of Shenyang Agricultural University， 2018， 49(6)： 686-696.
|
| [61] |
乌丽雅斯， 段爱国， 张建国 . 不同生境中亚沙棘亚种表型遗传分化规律研究[J]. 国际沙棘研究与开发， 2011， 9(1)： 16-23.
|
| [62] |
Wu Liyasi， Duan Aiguo， Zhang Jianguo. Study on phenotypic genetical diversity of H. rhamnoides subsp. turkestanica [J]. The Global Seabuckthorn Research and Development， 2011， 9(1)： 16-23.
|
| [63] |
黄铨， 佟金权， 杨培林， 吕志农 . 山西省关帝山中国沙棘种群表型结构的研究[J]. 林业科学研究， 1992， 5(5)： 512-517.
|
| [64] |
Huang Quan， Tong Jinquan， Yang Peilin， Lü Zhinong. Study on the population phenotype structure of Chinese Hippophae in Guandishan，Shanxi province [J]. Forest Research， 1992， 5(5)： 512-517.
|
| [65] |
尹世华， 王康， 黄晓霞， 李淑斌， 程小毛. 47 份月季品种表型多样性分析及综合评价[J]. 江西农业大学学报， 2021， 43(1)： 94-105.
|
| [66] |
Yin Shihua， Wang Kang， Huang Xiaoxia， Li Shubin， Cheng Xiaomao. Phenotypic diversity analysis and comprehensive evaluation of 47 rose resources[J]. Acta Agriculturae Universitatis Jiangxiensis， 2021， 43(1)： 94-105.
|
| [67] |
孙珍珠， 李秋月， 王小柯， 赵婉彤， 薛杨， 冯锦英， 刘小丰， 刘梦雨， 江东 . 宽皮柑橘种质资源表型多样性分析及综合评价[J]. 中国农业科学， 2017， 50(22)： 4362-4372.
|
| [68] |
Sun Zhenzhu， Li Qiuyue， Wang Xiaoke， Zhao Wantong， Xue Yang， Feng Jinying， Liu Xiaofeng， Liu Mengyu， Jiang Dong. Comprehensive evaluation and phenotypic diversity analysis of germplasm resources in Mandarin[J]. Scientia Agricultura Sinica， 2017， 50(22)： 4362-4372.
|
| [69] |
江锡兵， 滕国新， 范金根， 罗修宝， 盛建洪， 龚榜初 . 长江中下游区板栗主栽品种果实表型和品质综合评价[J]. 林业科学研究， 2022， 35(1)： 70-81.
|
| [70] |
Jiang Xibing， Teng Guoxin， Fan Jingen， Luo Xiubao， Sheng Jianhong， Gong Bangchu. Comprehensive evaluation of fruit phenotype and quality of main Chinese chestnut cultivars in the middle and lower reaches of the Yangtze River[J]. Forest Research， 2022， 35(1)： 70-81.
|
| [71] |
金争平， 蓝登明， 周世权 . 中国沙棘优良类型选育和俄罗斯大果沙棘引种研究[J]. 沙棘， 1998(4)： 10-16.
|
| [72] |
Jin Zhengping， Lan Dengming， Zhou Shiquan. Breeding of superior sea buckthorn Varieties in China and introduction of Russian large-fruit sea buckthorn[J]. Hippophae， 1998(4)： 10-16.
|
| [73] |
卢顺光， 卢健， 温秀凤 . 沙棘植物资源分布与营养学应用综述[J]. 中国水土保持， 2019(7)： 45-49.
|
| [74] |
Lu Shunguang， Lu Jian， Wen Xiufeng. Summary of distribution of seabuckthorn plant resources and application of nutrition[J]. Soil and Water Conservation in China， 2019(7)： 45-49.
|
| [75] |
安雄韬 . 不同品种大果沙棘果实品质研究[J]. 绿色科技， 2021， 23(3)： 78-80.
|
| [76] |
An Xiongtao. Study on fruit quality of different varieties of large berry sea buckthorn[J]. Journal of Green Science and Technology， 2021， 23(3)： 78-80.
|
基金资助
水利部沙棘良种选育试验示范项目(K9925026)
京公网安备11010202008535号
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