角蛋白多功能水凝胶的构建及其在糖尿病伤口愈合中的应用

Construction of keratin multifunctional hydrogel and its application in diabetic wound healing

摘要

过度氧化应激与细菌感染是糖尿病慢性伤口愈合过程中面临的两大重要难题,而现有兼具抗氧化与抗菌功能的水凝胶敷料往往依赖化学修饰,这可能损害其生物相容性且不利于临床应用。针对这些问题,以角蛋白、2-甲酰基苯硼酸(2-FPBA)和表没食子儿茶素没食子酸酯-锌(EGCG-Zn)复合物为原料,通过双动态交联反应,制备一种新型多功能水凝胶。其中,角蛋白与2-FPBA之间的巯基-醛基动态共价交联,以及EGCG-Zn复合物与2-FPBA之间的动态硼酸酯键共同构建了该水凝胶的网络结构。试验结果表明,该水凝胶能有效清除胞内活性氧,抑制大肠埃希菌和金黄色葡萄球菌的生长,显著加速糖尿病慢性伤口的愈合。该水凝胶制备过程简单,无需化学修饰,兼具优异的抗氧化与抗菌性能,在糖尿病慢性伤口治疗中展现出良好的应用前景。

Abstract

Excessive oxidative stress and bacterial infection are two major challenges in the healing process of chronic diabetic wounds. However, current hydrogel dressings with combined antioxidant and antibacterial functions often rely on chemical modifications, which may compromise their biocompatibility and hinder clinical application. To address these issues, a novel multifunctional hydrogel was prepared from keratin, 2-formylphenylboronic acid (2-FPBA), and epigallocatechin gallate-zinc (EGCG-Zn) complex through a double dynamic crosslinking reaction. Among them, the dynamic covalent crosslinking between the thiol group of keratin and the aldehyde group of 2-FPBA, as well as the dynamic borate ester bonds formed between the EGCG-Zn complex and 2-FPBA, jointly constructed the network structure of the hydrogel. Results show that this hydrogel can effectively scavenge intracellular reactive oxygen species, inhibit the growth of E.coli and S.aureus, and significantly accelerate the healing of chronic diabetic wounds. This hydrogel is simple to prepare, requires no chemical modification, and combines excellent antioxidant and antibacterial properties, showing promising potential for application in the treatment of chronic diabetic wounds.

关键词

多功能水凝胶 / 伤口愈合 / 抗氧化 / 抗菌

Key words

multifunctional hydrogel / wound healing / antioxidant / antibacterial

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基金资助

国家自然科学基金面上项目(32371413)