聚乙烯醇/可得然胶冷冻凝胶的制备及性能研究

Preparation and properties of poly(vinyl alcohol)/curdlan cryogel

摘要

冷冻凝胶因其高度互联的多孔结构、可调的力学性能及制备工艺简单的特性,在伤口敷料领域展现出重要的应用潜力。本研究以聚乙烯醇(PVA)和羧甲基化的可得然胶(CRc)为原料,通过冻融循环法制备PVA/CRc复合冷冻凝胶。研究了PVA与CRc的配比及冷冻循环系数对复合冷冻凝胶的理化性能及细胞相容性的影响。结果表明:CRc比例增加可显著扩大孔径(1PVA-3CRc:(20.43±4.48)μm)并提升孔隙率(1PVA-3CRc:(94.17±2.64)%)与含水率(1PVA-3CRc:(97.15±0.22)%);体外生物学评价证实,所有复合冷冻凝胶溶血率均低于5%,且凝血值随CRc比例增加呈剂量依赖性下降(从(37.60±7.10)%降至(7.97±3.25)%)。此外,相较于纯PVA,复合冷冻凝胶浸提液未显著影响L929的活力,但显著促进HUVECs的增殖,证明材料具有良好的细胞相容性。

Abstract

Cryogels have demonstrated significant potential for application in the field of wound dressings due to their highly interconnected porous structure, tunable mechanical properties and simple preparation process. In this study, PVA/CRc composite cryogels were prepared by freeze-thaw cycling method using polyvinyl alcohol (PVA) and carboxymethylated curdlan (CRc) as raw materials. The effects of the ratio of PVA to CRc and the freeze-thaw cycling coefficient on the physicochemical properties and cytocompatibility of the composite cryogels were investigated. The results show that an increase in the ratio of CRc significantly enlarges the pore size (1PVA-3CRc: (20.43±4.48) μm) and enhances the porosity (1PVA-3CRc:(94.17±2.64)%) and water content (1PVA-3CRc:(97.15±0.22)%). The in vitro biological evaluations confirmed that the haemolysis rate of all the composite cryogels was lower than 5%, and the coagulation values decreased in a dose-dependent manner with increasing CRc ratio (from (37.60±7.10)% to (7.97±3.25)%). In addition, the composite cryogel extract did not significantly affect the viability of L929 compared to pure PVA, but significantly promoted the proliferation of HUVECs, demonstrating the good cytocompatibility of the material.

关键词

聚乙烯醇 / 可得然胶 / 冷冻凝胶 / 伤口敷料 / 细胞相容性

Key words

polyvinyl alcohol / curdlan / cryogel / wound dressing / cytocompatibility

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基金资助

国家自然科学基金(32371383)

上海市科学技术委员会项目(23S31900100)

上海市徐汇区牙病防治所医学工程交叉项目(SHXYFYG202307)