载药铁碳核壳颗粒的制备与肿瘤细胞杀伤性能

Preparation of drug-loaded iron-carbon core-shell particles and their antitumor activity

摘要

核壳结构纳米颗粒具有独特的结构优势,能高效整合靶向递送、刺激响应释放以及成像等多功能,在疾病治疗领域引起广泛关注,但其制备仍面临诸多挑战。以二茂铁和过氧化氢为前驱体,通过热解法制备了多孔碳包裹的四氧化三铁(Fe₃O₄@C)核壳纳米颗粒,并利用磷脂表面改性提高其亲水性。在808 nm激光照射下,该纳米颗粒可将光能高效转换成热能,光热转换效率为32.7%。同时,光热效应能够显著提高芬顿反应速率,产生更多活性羟自由基。该颗粒具有优异的阿霉素(DOX)负载能力,负载效率为31.3%,并能实现温控药物快速释放。体外细胞试验证实Fe₃O₄@C的生物安全性。在808 nm激光照射下,负载DOX的Fe₃O₄@C纳米颗粒展现出高效的抗癌细胞效果。这种多功能纳米材料在肿瘤治疗领域具有重要的研究价值和应用前景。

Abstract

Core-shell structured nanoparticles possess unique structural advantages, enabling the effective integration of multiple functionalities such as targeted delivery, stimulus-responsive release, and imaging capabilities. These nanoparticles have attracted significant attention in the field of disease therapeutics, but their fabrication still faces substantial challenges. Using ferrocene and hydrogen peroxide as precursors , porous carbon-encapsulated ferroferric oxide (Fe₃O₄@C) core-shell nanoparticles were prepared through a pyrolysis method and modified with phospholipids to give them good hydrophilicity. Under 808 nm laser irradiation, the Fe₃O₄@C nanoparticles can efficiently convert light energy into thermal energy, with a photothermal conversion efficiency of 32.7%. Meanwhile, the photothermal effect can significantly enhance the Fenton reaction rate, generating more active hydroxyl radicals. In addition, the particles have excellent doxorubicin (DOX) loading capacity, with a loading efficiency of 31.3%, and achieve temperature-controlled drug release. In vitro cell experiments confirm biocompatibility of Fe₃O₄@C nanoparticles. Under 808 nm laser irradiation, the loading DOX-loaded Fe₃O₄@C nanoparticles exhibit efficient anti-cancer cell effects. The multifunctional nanoagents have important research value and application prospects in the field of tumor therapy.

关键词

核壳颗粒 / 载药 / 抗癌 / 光热转换 / 芬顿反应

Key words

core-shell particles / drug carrier / antitumor / photothermal conversion / Fenton reaction

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基金资助

国家重点研发计划(2021YFA1201304)