化学修饰对夏枯草多糖理化特征及生物活性的影响

Effects of Chemical Modification on Physicochemical Characteristics and Bioactivity of Prunella vulgaris Polysaccharide

摘要

采用超声破碎提取夏枯草多糖(PVPs),从中分离得到纯化多糖组分(PVP-2),并比较PVP-2及其锌、硒、硫酸化、磷酸化修饰PVP-2衍生物(Zn-PVP-2,Se-PVP-2,S-PVP-2和P-PVP-2)的理化特征、抗氧化、降血糖和抑菌活性.结果表明:与天然PVP-2相比,衍生物的糖醛酸、总酚、总黄酮、硫酸基团、葡萄糖醛酸和半乳糖醛酸的含量、水溶性、抗氧化、降血糖及抑菌活性均明显提高,但相对分子质量和蛋白质含量降低.修饰导致PVPs的中性侧链降解,并明显改变了单糖的物质的量比,但未改变PVP-2的主链结构.P-PVP-2的抗氧化活性和降血糖活性最高,Se-PVP-2的抑菌活性最好,表明化学修饰方法对PVP-2的修饰均有效,化学修饰可提升PVPs在食品和医药工业中的应用潜力.

Abstract

Prunella vulgaris polysaccharides (PVPs) were extracted by using ultrasonication extraction,and polysaccharide component (PVP-2) was isolated and purified from PVPs. Physicochemical characteristics,antioxidant,hypoglycemic and antibacterial activities of PVP-2 and derivatives(Zn-PVP-2,Se-PVP-2,S-PVP-2 and P-PVP-2)modified by zinc,selenium,sulfonation and phosphorylation were compared. The results show that compared with natural PVP-2,the contents of uronic acid,total phenol,total flavonoids,sulfuric acid group,glucuronic acid and galacturonic acid,water solubility,antioxidant,hypoglycemic and antibacterial activities of the derivatives are significantly increased,but the relative molecular weight and protein content are decreased. The modification leads to the degradation of the neutral side chains of PVPs,and significantly changes the molar ratio of monosaccharides,but does not change the backbone structure of PVP-2. P-PVP-2 has the highest antioxidant activity and hypoglycemic activity,and Se-PVP-2 has the best antibacterial activity,indicating that the chemical modification methods are effective in the modification of PVP-2,and chemical modification can enhance the potential application of PVPs in the food and pharmaceutical industries.

关键词

夏枯草多糖 / 修饰 / 理化特征 / 生物活性

Key words

Prunella vulgaris polysaccharide / modification / physicochemical characteristics / bioactivity

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基金资助

吉林省自然科学基金(YDZJ202101ZYTS183)