海洋真菌Talaromyces sp. UJNMF0655代谢产物的结构鉴定与生物活性

Structures and bioactivities of metabolites of marine fungus Talaromyces sp. UJNMF0655

摘要

目的 研究海榄雌Avicennia marina(Forssk.)Vierh.根部沉积物来源真菌Talaromyces sp. UJNMF0655的次级代谢产物及其生物活性。 方法 采用正相硅胶色谱、反相硅胶色谱、Sephadex凝胶色谱、高效液相色谱等方法对目标真菌发酵物的乙酸乙酯提取物进行分离纯化,结合化合物的理化性质、波谱数据、文献报道等进行结构鉴定,并评价其抗菌、神经保护、抗氧化活性。 结果 从海洋真菌Talaromyces sp. UJNMF0655的次级代谢产物中分离鉴定了9个聚酮类化合物:talaroisochromane A(1)、talaroisochromane B(2)、penicillide(3)、paecilin L(4)、paecilin E(5)、paecilin N(6)、paecilin P(7)、paecilin F(8)、paecilin A(9),其中8个化合物(1-2,4-9)为二聚体结构,化合物1和2是新化合物。活性结果显示,化合物9对S. aureus有抑菌活性,化合物3在50 μmol/L时能够明显减少高糖引起的RSC96细胞损伤。 结论 海洋真菌Talaromyces sp. UJNMF0655能够产生丰富的二聚体类成分,说明该菌具有开发抗菌和神经保护先导化合物的潜力。

Abstract

Objective To investigate the secondary metabolites and their bioactivities of Talaromyces sp. UJNMF0655, a fungus isolated from the root sediment of the mangrove Avicennia marina(Forssk.)Vierh. Methods The ethyl acetate extract of the target fungus fermentation broth was isolated and purified using various chromatographic techniques, including normal-phase silica gel chromatography, reversed-phase silica gel chromatography, Sephadex gel chromatography, and high-performance liquid chromatography(HPLC). The structures of the isolated compounds were elucidated through comprehensive analysis of their physicochemical properties, spectroscopic data, and comparison with literature reports, while their antimicrobial, neuroprotective, and antioxidant activities were evaluated. Results Nine polyketide compounds were isolated and identified from the secondary metabolites of the marine fungus Talaromyces sp. UJNMF0655: talaroisochromane A(1), talaroisochromane B(2), penicillide(3), paecilin L(4), paecilin E(5), paecilin N(6), paecilin P(7), paecilin F(8), and paecilin A(9). Among these, eight compounds(1-2, 4-9)featured dimeric structures, and compounds 1 and 2 were new natural products. Bioactivity results indicated that compound 9 exhibited certain antibacterial activity against S. aureus, and compound 3, at a concentration of 50 μmol/L, significantly reduced high glucose-induced damage in RSC96 cells. Conclusion The marine fungus Talaromyces sp. UJNMF0655 is capable of producing abundant dimeric metabolites, demonstrating the potential of UJNMF0655 to develop lead compounds with antimicrobial and neuroprotective activities.

关键词

海洋真菌 / Talaromyces sp. UJNMF0655 / 代谢产物 / 二聚色原酮 / 生物活性

Key words

Marine fungus / Talaromyces sp. UJNMF0655 / Metabolites / Dimeric chromanones / Bioactivity

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基金资助

国家自然科学基金(82273819)

山东省高等学校青年创新团队发展计划项目(2022KJ096)