不同百合种质资源对灰葡萄孢菌的抗性鉴定及生理生化指标分析

Resistance and physiological-biochemical analysis of Lilium to Botrytis cinerea

摘要

【目的】探讨不同百合种质资源抗灰霉病的生理机制。【方法】以部分野生百合和栽培百合为试材，以灰葡萄孢（Botrytis cinerea）为供试菌株，测定不同百合离体叶片接菌后的灰霉病抗性，以及8种抗性不同的栽培百合中抗氧化酶活性、丙二醛（MDA）含量、活性氧物质含量和渗透调节物质含量等9个胁迫因子指标。【结果】在15种野生百合中筛选出7种抗性资源；栽培百合A型和LA杂交系列为感病类型，O型和OT系列为抗病类型。超氧化物歧化酶（SOD）和过氧化物酶（POD）活性在百合抗病过程中起关键作用；接菌后48 h的MDA含量、超氧阴离子（O₂-·）含量及其变化幅度可作为筛选百合抗病性的指标。【结论】抗病百合通过调控活性氧（ROS）水平和渗透调节物质含量，实现对灰霉病的防御。

Abstract

[Objective] This study aimed to explore the physiological mechanisms underlying resistance to gray mold in diverse lily germplasms. [Method] Using several wild and cultivated lily varieties as experimental materials and Botrytis cinerea as the test strain, gray mold resistance of detached lily leaves after inoculation was assessed, along with antioxidant enzyme activities and the contents of malondialdehyde (MDA), reactive oxygen species (ROS), and osmotic adjustment substances in eight cultivated lily varieties exhibiting varying levels of resistance. [Result] Seven resistant resources were identified among fifteen wild lily species. Cultivated lilies of the A and LA hybrid series were susceptible, whereas those of the O and OT series were resistant. Superoxide dismutase (SOD) and peroxidase (POD) activities were critical for lily resistance to gray mold; MDA content, superoxide anion radical (O₂-·) content, and their variation ranges at 48 h post-inoculation served as indicators for screening disease-resistant lilies. [Conclusion] Disease-resistant lilies defend against gray mold by regulating ROS levels and osmotic adjustment substance contents.

关键词

百合 / 灰葡萄孢菌 / 抗病性 / 生理生化指标 / 抗病机制

Key words

Lily / Botrytis cinerea / disease resistance / physiological and biochemical indicators / resistance mechanism

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基金资助

云南省基础研究专项面上项目(202401AT070152)

国家自然科学基金项目(32460772)

云南省农业科学院人才引培计划(2024RCYP-08)

云南省花卉育种重点实验室开放基金项目(FKL-202303)