黄瓜 miR166 家族特征及其在干旱和盐胁迫下的表达分析

Analysis of the characteristics and expression patterns of the miR166 family in cucumber under drought and salt stress conditions

摘要

【目的】解析黄瓜miR166家族成员的特性以及在干旱和盐胁迫下的表达模式，为黄瓜miR166家族成员的功能研究提供理论依据。【方法】基于miRNA测序数据，系统地鉴定黄瓜miR166家族成员（miR166s）并分析其特征特性，利用miRNA测序数据和RT-qPCR技术分析黄瓜miR166s在干旱和盐胁迫下的表达情况。【结果】15个miR166s聚类在3个进化分支上，分布于黄瓜5条染色体上，且成熟体序列在系统发育中相对保守。多数miR166s在前期响应干旱胁迫且相对稳定，部分miR166s在盐胁迫下大量表达或超高表达，如MIR166c-p5在盐胁迫下超高表达，miR166e_L+2R-1、miR166a-3p_R+2在盐胁迫下大量表达，说明miR166s家族成员在黄瓜响应干旱和盐胁迫过程中可能发挥重要作用。【结论】miR166家族成员参与黄瓜响应干旱和盐胁迫过程。

Abstract

[Objective] This study aimed to analyze the characteristics of cucumber miR166 family members and their expression patterns under drought and salt stress, providing a theoretical basis for functional studies of cucumber miR166 family members. [Method] Based on miRNA sequencing data, cucumber miR166 family members (miR166s) were systematically identified, and their characteristic features were analyzed. miRNA sequencing data and RT-qPCR techniques were used to analyze the expression of cucumber miR166s under drought and salt stress. [Result] Fifteen miR166s were clustered into three evolutionary branches and distributed across five cucumber chromosomes, with their mature sequences being relatively conserved in phylogeny. Most miR166s responded to drought stress at early stages with relatively stable expression levels, while some miR166s showed high or extremely high expression under salt stress. For example, MIR166c-p5 exhibited extremely high expression under salt stress, and miR166e_L+2R-1 and miR166a-3p_R+2 were highly expressed under salt stress, indicating that miR166 family members may play important roles in cucumber's response to drought and salt stress. [Conclusion] miR166 family members are involved in cucumber's response to drought and salt stress processes.

关键词

黄瓜 / miR166 / 生物信息学 / 非生物胁迫 / RT-qPCR

Key words

cucumber / miR166 / bioinformatics / abiotic stress / RT-qPCR

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基金资助

安徽省自然科学研究重点项目(2023AH051056)