马铃薯抗晚疫病及病毒病种质资源分子标记辅助筛选

Molecular Marker-assisted Screening of Potato Germplasm Resources for Resistance to Late Blight and Virus

摘要

为筛选具有多个晚疫病、马铃薯Y病毒（Potato virus Y，PVY）和马铃薯卷叶病毒（Potato leafroll virus，PLRV）抗性基因的马铃薯种质资源，利用4个与晚疫病抗性相关分子标记R2-800、R3a、R3b、R2F，3个与抗PVY的基因Rysto、Rychc紧密连锁的分子标记YES3-3B、GP122-564、Ry364和抗PLRV的基因PLRV.1紧密连锁的分子标记N1271164，对174份山西省保存的马铃薯种质资源进行检测。174份种质资源均含有1个或1个以上的抗性分子标记，单个标记中含有R3b和GP122-564抗性分子标记的有173份；多个标记中只含有7个标记R3a、R3b、R2F、N1271164、YES3-3B、Ry364、GP122-564的马铃薯种质资源有84份。聚类分析结果将174份种质资源分为5类，其中第III类25份均含有8个分子标记，其中仅有6份为国内育成品种，分别为‘早大白’ ‘内薯7号’ ‘希森4号’ ‘天薯9号’ ‘中薯19号’ ‘京张薯1号’，其余均为国际马铃薯中心提供的资源，因此这一类可作为山西省马铃薯抗病育种材料。筛选出的晚疫病和PVY、PLRV抗性资源，将为相关病害抗性资源、分子标记的进一步利用以及抗病基因的聚合育种奠定基础。

Abstract

This study utilized four molecular markers R2-800, R3a, R3b, and R2F associated with late blight resistance, three molecular markers YES3-3B, GP122-564, and Ry364 closely linked to the Rysto and Rychc genes for potato virus Y (PVY) resistance, and molecular marker N1271164 closely linked to potato leafroll virus (PLRV) resistance to test 174 potato germplasm resources conserved in Shanxi Province, in order to screen potato germplasm resources with multiple late blight, PVY and PLRV resistant genes. All of 174 germplasm resources contained one or more resistant molecular markers, and 173 contained the R3b and GP122-564 resistant markers in a individual markers. Furthermore, 84 potato germplasm resources contained seven markers, R3a, R3b, R2F, N1271164, YES3-3B, Ry364, and GP122-564. Cluster analysis classified the 174 germplasm resources into five distinct classes. Notably, 25 entries in Class III contained eight molecular markers, of which only six were domestic varieties, namely 'Zaodabai', 'Neishu 7', 'Xisen 4', 'Tianshu 9', 'Zhongshu 19', and 'Jingzhangshu 1', and the remaining germplasm resources were provided by the International Potato Center. Therefore, this category could be used as a valuable source for breeding disease-resistant potatoes in Shanxi Province. The screening of a number of late blight and PVY and PLRV resistant resources would lay the foundation for the further utilization of related disease resistant resources and molecular markers as well as the polymerization of disease resistant genes for breeding.

关键词

马铃薯 / 分子标记 / 晚疫病 / 马铃薯Y病毒（PVY） / 马铃薯卷叶病毒（PLRV）

Key words

potato / molecular marker / late blight / potato virus Y (PVY) / potato leafroll virus (PLRV)

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基金资助

国家马铃薯产业技术体系(CARS-09-ES02)

山西省高等学校科技创新项目(2023L038)

山西农业大学生物育种工程(YZGC082)

山西省马铃薯产业技术体系种质资源创制岗位(CYJSTX06-04)