听觉皮层双耳整合神经元的层响应分布特异性及突触输入调控机制

Layer-specific response distribution characteristics of binaural integration neurons in the auditory cortex and synaptic input regulation mechanism

摘要

目的 探究小鼠初级听觉皮层神经元对不同侧耳纯音刺激的响应特性及兴奋/抑制输入特性。方法 采用光遗传学结合在体清醒动物细胞外记录技术，给予小鼠不同强度和频率纯音组合，并随机通过对侧耳、同侧耳和双侧耳给声；同时，结合离体脑片全细胞记录技术，探究听觉皮层深层和第四层(L4)神经元的兴奋/抑制输入特性。结果 A1 L4神经元对双侧耳刺激表现为增益效应，而深层神经元则表现为抑制效应。L4兴奋性神经元接收来自同侧深层的输入时，以接收兴奋性输入为主。A1深层兴奋性神经元接收来自对侧输入时，以接收抑制性输入为主。结论 小鼠A1神经元对不同侧耳纯音刺激展现出差异化的响应特性，不同层神经元在接收同侧或对侧输入时，其兴奋/抑制输入特性存在明显差异。这些发现为深入解析听觉皮层复杂的双耳整合机制提供了新的视角和理论依据。

Abstract

Objective To investigate the response characteristics of neurons in the primary auditory cortex (A1) of mice to pure tone stimuli from different sides of the ear, as well as their excitatory/inhibitory input properties. Methods Optogenetics combined with in vivo extracellular recordings in awake mice was used to present pure tone stimuli of varying intensities and frequencies, delivered randomly to the contralateral ear, ipsilateral ear, or both ears. In addition, optogenetics together with in vivo brain slice whole-cell recordings was employed to investigate the excitatory and inhibitory input properties of deep-layer neurons and layer 4 (L4) neurons in the auditory cortex. Results A1 L4 neurons exhibited a gain effect in response to bilateral ear stimulation, while deep-layer neurons exhibited an inhibitory effect. When L4 excitatory neurons received input from the ipsilateral deep layers, they primarily reveived excitatory input. When A1 deep excitatory neurons reveived input from the contralateral side, they primarily reveived inhibitory input. Conclusion Mouse A1 neurons exhibit distinct response properties to pure tone stimuli from different sides of ear. Neurons in different layers show significant differences in their excitatory/inhibitory input characteristics when receiving inputs from the ipsilateral or contralateral side. These findings provide new insights and theoretical foundations for further understanding the complex binaural integration mechanisms in the auditory cortex.

关键词

听觉皮层 / 兴奋/抑制 / 层响应 / 纯音 / 光遗传学

Key words

Auditory cortex / Excitation/inhibition / Layer response / Pure tone / Optogenetics

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基金资助

广东省自然科学基金-面上项目(2024A1515012279)

广东省自然科学基金-面上项目(2017A030313186)