眼局部微压氧对兔角膜上皮细胞损伤后修复及氧化应激的影响

The effect of local micro-pressure oxygen on the repair and oxidative stress of rabbit corneal epithelial cells after injury

摘要

目的 探讨眼局部微压氧对兔角膜上皮损伤后修复及微观结构变化的影响，并分析其对角膜氧化应激水平的影响。方法 选取健康成年新西兰大白兔75只(150只眼)，建立角膜上皮损伤模型。随机分为A、B、C三组，每组25只(50只眼)。A组给予局部微压氧治疗(5 L/min；浓度:95%；1.5±0.5 kPa)，供氧2 h/d；B组给予普通无压力氧气治疗(5 L/min；浓度:95%)，供氧2 h/d；C组自然愈合。透射电镜观察各组角膜上皮微观结构变化。使用Image-J软件测量角膜上皮未愈合面积。使用ELISA法检测角膜氧化应激因子丙二醛的浓度变化。结果 造模后12、24、36 h，三组角膜上皮未愈合面积存在组间与组内的交互作用且组间不同时间点总体比较差异存在统计学意义(F_时间=839828.012，P<0.001；F_组别=38866.823，P<0.001；F_{时间*组别}=7338.970，P<0.001)，三组角膜上皮未愈合面积均为A组<B组<C组。透射电镜结果显示，造模后36 h，相较于B、C两组，A组的角膜上皮细胞结构更为完整。造模后12、24、36 h，三组丙二醛浓度组别与时间的交互作用不显著且组间不同时间点总体比较均不存在统计学意义(F_组间=0.172，P=0.843；F_{时间*组别}=1.572，P_{时间*组别}=0.226)。结论 眼局部微压氧治疗可有效缩短角膜上皮损伤后的修复时间，且角膜的氧化应激水平无明显改变。

Abstract

Objective To investigate the impact of topical microbaric oxygen therapy on corneal epithelial wound healing and ultrastructural alterations in rabbits, and to elucidate its modulatory effects on corneal oxidative stress levels. Methods A total of 75 healthy adult New Zealand White rabbits (150 eyes) were enrolled to establish a corneal epithelial defect model. Animals were randomly assigned to three groups (n=25 rabbits/50 eyes each). Group A received topical microbaric oxygen therapy for 2 h daily; Group B received normobaric oxygen at identical flow and concentration for the same duration; Group C was left to heal spontaneously. Corneal epithelial ultrastructure was evaluated by transmission electron microscopy. Residual epithelial defects were planimetrically quantified with Image-J software. Corneal oxidative stress was assessed by measuring malondialdehyde concentration via enzyme-linked immunosorbent assay. Results At 12, 24, and 36 h post-injury, a significant interaction between group and time was detected for the residual corneal epithelial defect area (F_time=839,828.012, P<0.001; F_group=38,866.823, P<0.001; F_time*group=7,338.970, P<0.001); the defect consistently ranked A<B<C across all intervals. Transmission electron microscopy at 36 h revealed better-preserved ultrastructural integrity of corneal epithelial cells in group A relative to groups B and C. In contrast, no significant group-time interaction or inter-group differences were observed for malondialdehyde concentration at any time point (F_group=0.172, P=0.843; F_time*group=1.572, P=0.226). Conclusion Topical microbaric oxygen therapy significantly accelerates corneal epithelial wound closure without inducing a measurable increase in corneal oxidative stress.

关键词

眼局部微压氧 / 角膜上皮细胞 / 修复 / 氧化应激 / 透射电镜

Key words

Topical microbaric oxygen therapy / Corneal epithelial cells / repair / Oxidative stress / Transmission electron microscope

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基金资助

济南市卫生健康委科技发展计划项目(2025105001)

济南高层次人才项目(CYLJ20241901459)

济南市临床医学科技创新计划(202225058)