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摘要
类萌发素蛋白（germin-like proteins,GLPs）是一类高度保守的胁迫响应蛋白，可特异性响应菌根共生。该研究基于玉米（Zea mays）B73全基因组信息，利用生物信息学方法对ZmGLP基因家族成员进行鉴定及特征分析，利用转录组数据分析ZmGLP基因在丛枝菌根真菌（AMF）共生下的表达模式。该研究鉴定了45个ZmGLP基因，它们分布于9条染色体上，存在25个串联复制基因；系统发育树将ZmGLP基因分为5个亚家族；不同ZmGLP基因组织表达模式存在较大差异。启动子顺式作用元件分析显示，ZmGLP基因启动子包含响应光、胁迫和生长发育相关的元件，ZmGLP4-8启动子含有菌根响应元件MYCS,20个ZmGLP基因启动子包含潜在的菌根响应元件GCCGGC；基于接种AMF后不同时间的玉米根部转录组数据，发现12个ZmGLP基因的表达在接种AMF后出现了显著变化；其中ZmGLP3-3、ZmGLP4-8、ZmGLP4-16、ZmGLP4-20、ZmGLP5-1、ZmGLP6-1在共生后期显著上调表达，且与已报道的共生相关GLP基因在不同的进化分支，暗示这些基因可能参与菌根共生后期相关的功能。ZmGLP3-3功能研究显示，相较于野生型玉米植株，突变体zmglp3-3菌根定殖率显著降低。综上，该研究为共生相关ZmGLP基因挖掘提供了理论基础。
关键词
丛枝菌根真菌
/
类萌发素蛋白
/
基因家族
/
菌根共生
/
表达分析
Key words
arbuscular mycorrhizal fungi
/
germin-like proteins
/
gene family
/
mycorrhizal symbiosis
/
expression analysis
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春建惠,董文龙,屠元超,刘芳,徐云剑.
玉米GLP家族基因鉴定及其响应丛枝菌根共生表达[J].
植物研究, 2025, 45(03): 406-418 DOI:10.7525/j.issn.1673-5102.2025.03.011
基金资助
云南省科技计划项目(202401BF070001-002)
云南省科技计划项目(202301AT070106)
云南省教育厅科学研究基金项目(KC-24248854)
京公网安备11010202008535号
