miR-21、miR-26a及miR-200a在子痫前期患者中的表达水平及与病情的相关性

Expression levels of miR-21, miR-26a and miR-200a in patients with preeclampsia and correlation with the condition

摘要

目的 分析miR-21、miR-26a及miR-200a在子痫前期(PE)患者血清中的表达,并探讨其临床意义。方法 选取2019年3月至2021年3月在南京市妇幼保健院治疗的PE患者103例,分为轻度PE组(57例)和重度PE组(46例),另选取同期正常孕妇50例作为对照组.检测各组孕妇血清miR-21、miR-26a及miR-200a表达水平,分析其与年龄、孕周、BMI、血压、24h尿蛋白及不良妊娠结局的相关性,并分析血清miR-21、miR-26a及miR-200a对PE的诊断价值。结果 重度PE组BMI、舒张压、收缩压以及24h尿蛋白高于轻度PE组及对照组,且差异具有统计学意义(F值分别为4.116、141.063、143.118和342.736,P<0.05).对照组、轻度PE组及重度PE组孕妇血清中miR-21、miR-26a及miR-200a的表达水平依次升高.Pearson相关性分析结果显示,血清miR-21、miR-26a及miR-200a的表达水平与血压、24h尿蛋白及不良妊娠结局发生率呈正相关(r值介于0.446~0.832之间,P<0.001).经受试者工作特征曲线(ROC曲线)分析发现,血清miR-21、miR-26a及miR-200a联合诊断PE的曲线下面积(AUC)为0.967,敏感度为98.10%,特异性为92.30%。结论 PE患者血清miR-21、miR-26a及miR-200a的表达高于正常孕妇,且与患者的临床指标及不良妊娠结局相关,检测血清miR-21、miR-26a、miR-200a水平对早期诊断PE及评估患者预后具有十分重要的意义.

Abstract

Objective To analyze the expression of miR-21, miR-26a and miR-200a in serum of patients with preeclampsia (PE), and to explore their clinical significance. Methods A total of 103 PE patients who treated in Nanjing Women and Children's Healthcare Hospital from March 2019 to March 2021 were selected and divided into mild PE group (57 cases) and severe PE group (46 cases), and another 50 normal pregnant women in the same period were selected as control group. The expression levels of miR-21, miR-26a and miR-200a in serum of pregnant women in each group were detected, and their correlation with age, gestational week, BMI, blood pressure, 24h urinary protein and adverse pregnancy outcomes were analyzed, as well as the diagnostic value of serum miR-21, miR-26a and miR-200a for PE. Results BMI, diastolic blood pressure, systolic blood pressure, and 24-h urinary protein in the severe PE group were higher than those in the mild PE group and the control group, and the differences were statistically significant (F=4.116, 141.063, 143.118, and 342.736, respectively, P<0.05). The expression levels of miR-21, miR-26a and miR-200a in the serum of pregnant women in control group, mild PE group and severe PE group were increased sequentially. Pearson correlation analysis showed that the expression levels of serum miR-21, miR-26a and miR-200a were positively correlated with blood pressure, 24-hour urinary protein and the incidence of adverse pregnancy outcomes (r values ranged from 0.446 to 0.832, P<0.001). The area under the curve (AUC) for the combined diagnosis of PE with serum miR-21, miR-26a and miR-200a was 0.967, with a sensitivity of 98.10% and a specificity of 92.30%, as analyzed by the receiver operating characteristic (ROC) curve of the subjects. Conclusion The expression of serum miR-21, miR-26a and miR-200a in PE patients is higher than that in normal pregnant women, and it is related to clinical indicators and adverse pregnancy outcomes. Detection of serum miR-21, miR-26a and miR-200a levels is of great significance for early diagnosis and prognosis of PE patients.

关键词

miR-21 / miR-26a / miR-200a / 子痫前期 / 早期诊断

Key words

miR-21 / miR-26a / miR-200a / preeclampsia / early diagnosis

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基金资助

南通市科技项目(JC2021045)