宫颈癌组织FMNL3、ASAP1表达与患者临床病理特征、预后的关系

Expression of FMNL3 and ASAP1 in cervical cancer tissues and the relationship with clinicopathological characteristics and prognosis

摘要

目的 探究宫颈癌(CCA)组织成蛋白样3型蛋白(FMNL3)、ADP核糖基化因子的GTP酶激活蛋白1(ASAP1)的表达及其与患者临床病理特征、预后的关系。方法 选择2016年9月至2019年8月在铜川市人民医院诊治的85例CCA患者,取术中保存完好的CCA组织和癌旁组织。免疫组织化学染色法(IHC)检测FMNL3、ASAP1表达情况;χ²检验分析FMNL3、ASAP1表达与临床病理特征的关系;Spearman相关分析FMNL3、ASAP1的相关性;COX多因素回归分析CCA患者术后生存预后的影响因素;Kaplan-Meier法进行生存分析,组间生存曲线差异用对数秩检验(log rank test)。结果 FMNL3、ASAP1在CCA组织中阳性表达率高于在癌旁组织中的阳性表达率(χ²=27.234、28.888,P<0.001);FMNL3和ASAP1在CCA组织中的表达呈正相关性(r=0.495,P<0.001);FMNL3、ASAP1表达情况与浸润深度、淋巴结转移、TNM分期、组织分化程度、远处转移有关(χ²介于4.284到10.983之间,P<0.05);FMNL3阳性表达患者5年生存率(43.10%)低于阴性表达患者(84.48%)(log rank χ²=12.118,P<0.001),ASAP1阳性表达患者5年生存率(45.16%)低于阴性表达患者(82.61%)(Log rank χ²=9.694,P=0.002);浸润深度(>1/2)、TNM分期(Ⅱ期)、组织分化程度(中低分化)、远处转移(有)及FMNL3、ASAP1阳性表达是CCA患者术后5年内死亡的独立危险因素(HR介于1.286到1.986之间,P<0.05)。结论 FMNL3、ASAP1在CCA组织中阳性表达,并呈正相关。两者表达与部分临床病理特征有关,阳性表达患者5年生存率较低,是患者死亡的独立危险因素。

Abstract

Objective To investigate the expression of formin like-3 (FMNL3) and ADP ribosylation factor GTPase activating protein 1 (ASAP1) in cervical cancer (CCA) tissues, and to analyze their associations with clinicopathological characteristics and patient prognosis. Methods A total of 85 patients with CCA treated at Tongchuan People's Hospital from September 2016 to August 2019 were included. Well-preserved CCA tissues and adjacent non-cancerous tissues obtained during surgery were collected. Immunohistochemistry (IHC) was used to detect FMNL3 and ASAP1 expression. The χ² test was applied to analyze correlations between FMNL3/ASAP1 expression and clinicopathological features. Spearman correlation analysis was conducted to evaluate the relationship between FMNL3 and ASAP1 expression. Cox multivariate regression analysis was performed to identify prognostic factors influencing postoperative survival in CCA patients. Kaplan-Meier survival analysis was used to estimate survival rates, and intergroup differences were tested using the log rank test. Results The positive expression rates of FMNL3 and ASAP1 were significantly higher in CCA tissues than in adjacent tissues (χ²=27.234 and 28.888, respectively, P<0.001). A positive correlation was observed between FMNL3 and ASAP1 expression in CCA tissues (r=0.495, P<0.001). Expression levels of FMNL3 and ASAP1 were significantly associated with depth of invasion, lymph node metastasis, TNM stage, histological differentiation, and distant metastasis (χ²=4.284-10.983, P<0.05). Patients with positive FMNL3 expression had a lower 5-year survival rate (43.10%) compared with those with negative expression (84.48%) (log rank χ²=12.118, P<0.001). Similarly, ASAP1-positive patients had reduced 5-year survival (45.16%) versus ASAP1-negative subjects (82.61%) (log rank χ²=9.694, P=0.002). Depth of invasion (>1/2), TNM stage (stage Ⅱ), poor/moderate differentiation, presence of distant metastasis, and positive expression of FMNL3 and ASAP1 were identified as independent risk factors for mortality within 5 years post-surgery (HR=1.286-1.986, P<0.05). Conclusion FMNL3 and ASAP1 are positively expressed in CCA tissues and show a positive correlation. Their expression is associated with specific clinicopathological characteristics, and positive expression predicts poorer 5-year survival, serving as independent risk factors for mortality in CCA patients.

关键词

宫颈癌 / 临床病理特征 / 成蛋白样3型蛋白 / ADP核糖基化因子的GTP酶激活蛋白1 / 预后

Key words

cervical cancer / clinicopathological characteristic / formin like-3 / ADP ribosylation factor GTPase activating protein 1 / prognosis

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基金资助

陕西省重点研发计划项目(2019SFG116)