环状RNA hsa_circ_0085576调控微小RNA-498/B细胞特异性莫洛尼鼠白血病病毒整合位点1轴对口腔鳞状细胞癌细胞迁移和侵袭的影响

Effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma by regulating the microRNA-498/B-cell-specific Moloney murine leukemia virus integration site 1 axis

摘要

目的 探究环状RNA hsa_circ_0085576对口腔鳞状细胞癌（OSCC）细胞迁移和侵袭的影响及其分子机制。 方法 使用实时荧光定量聚合酶链反应（qRT-PCR）和蛋白印迹法检测OSCC细胞中hsa_circ_0085576、微小RNA-498（miR-498）以及B细胞特异性莫洛尼鼠白血病病毒整合位点1（BMI-1）的表达水平。使用CCK-8、划痕实验、Transwell实验以及qRT-PCR、蛋白印迹法分别检测SCC-15细胞增殖活力、迁移及侵袭能力以及相关基因和蛋白的相对表达量。 结果 OSCC细胞中hsa_circ_0085576与BMI-1表达上调，miR-498表达下调（P<0.05）。下调hsa_circ_0085576表达或过表达miR-498后SCC-15细胞的增殖活性、划痕愈合率、侵袭细胞数目以及细胞周期蛋白D1、波形蛋白表达水平下调，miR-498和E-钙黏蛋白表达水平上调（P<0.05）；抑制miR-498表达可减弱下调hsa_circ_0085576表达对OSCC细胞增殖、迁移及侵袭的抑制作用；上调BMI-1表达可减弱过表达miR-498对OSCC细胞增殖、迁移及侵袭的抑制作用。 结论 下调hsa_circ_0085576表达可通过激活miR-498/BMI-1轴抑制OSCC细胞增殖、迁移及侵袭。

Abstract

Objective This study aims to explore the effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma (OSCC) and the underlying molecular mechanism. Methods Circular RNA hsa_circ_0085576, microRNA-498 (miR-498), and B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) in the cells of OSCC were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses. CCK-8, scratch test, Transwell test, qRT-PCR, and Western blot were used to detect the proliferation, migration, and invasion ability of SCC-15 cells and the expression of related genes and proteins. Results The expression of hsa_circ_0085576 and BMI-1 in the cells of OSCC was upregulated, and that of miR-498 was downregulated (P<0.05). The proliferative activity, scratch healing rate, number of invasive cells, and expression levels of cyclinD1 and vimentin proteins of SCC-15 cells were downregulated, whereas the expression levels of miR-498 and E-cadherin proteins were upregulated (P<0.05). Inhibition of miR-498 expression weakened the inhibitory effect of downregulating hsa_circ_0085576 on the proliferation, migration, and invasion of OSCC cells. Upregulation of BMI-1 expression attenuated the inhibitory effect of overexpressed miR-498 on the proliferation, migration, and invasion of OSCC cells. Conclusion Downregulation of hsa_circ_0085576 expression inhibited the proliferation, migration, and invasion of OSCC cells by activating the miR-498/BMI-1 axis.

关键词

环状RNA hsa_circ_0085576 / 微小RNA-498 / B细胞特异性莫洛尼鼠白血病病毒整合位点1 / 口腔鳞状细胞癌

Key words

circular RNA hsa_circ_0085576 / microRNA-498 / B-cell-specific Moloney murine leukemia virus integration site 1 / oral squamous cell carcinoma

Author summay

李立恒，主治医师，硕士，Email：[e-mail]