Toll样受体和白细胞介素-17单核苷酸多态性与口腔扁平苔藓易感性的相关性研究

The correlation study between single nucleotide polymorphisms of Toll-like receptor and interleukin-17 and oral lichen planus

摘要

目的 探讨Toll样受体（TLR）和白细胞介素-17（IL-17）基因的遗传多态性及与口腔扁平苔藓（OLP）的相关性。 方法 选取病例组（OLP组）患者与对照组（未患OLP人群）外周血提取的DNA为研究对象，选取TLR与IL-17上的7个单核苷酸多态性（SNP）位点，分别是TLR3中的rs5743312、TLR2中的rs121917864和IL-17A中的5个SNP位点（rs4711998、rs3819024、rs8193036、rs3748067和rs17878530）。 采用高分辨率熔解曲线分析法进行外周血细胞PCR产物基因多态性检测，并与DNA测序结果比对。统计分析SNP位点与OLP发生的相关性。 结果 rs5743312（TLR3）、rs3819024（IL-17A）与OLP的发生相关，并有统计学意义（P<0.05）。 其余5个SNP位点未检测出与OLP相关。 结论 rs5743312（TLR3）和rs3819024（IL-17A）可能与OLP的易感性相关。

Abstract

Objective This study aimed to explore the genetic polymorphisms of the Toll-like receptor (TLR) and interleukin (IL)-17 genes and their correlation with oral lichen planus (OLP). Methods DNA extracted from the peripheral blood of patients in the case (OLP group) and control (non-OLP population) groups were selected as the research subjects. Seven single nucleotide polymorphisms were attempted in our study, namely, rs5743312 in TLR3, rs121917864 in TLR2, and five others (rs4711998, rs3819024, rs8193036, rs3748067, and rs17878530) in IL-17A. High resolution melting curve analysis was used to detect the gene polymorphism of PCR products in peripheral blood cells and compared with DNA sequencing. The correlation between polymorphic sites and the occurrence of OLP was statistically analyzed. Results We found that rs5743312 (TLR3) and rs3819024 (IL-17A) had a statistically significant association with OLP (P<0.05). The remaining five SNPs cannot be tested for association with OLP. Conclusion rs5743312 (TLR3) and rs-3819024 (IL-17A) may contribute to the initiation of OLP.

关键词

单核苷酸多态性 / 高分辨率熔解曲线分析 / 口腔扁平苔藓 / 易感性

Key words

single nucleotide polymorphism / high resolution curve melting analysis / oral lichen planus / susceptibility

Author summay

李然，副教授，博士，Email：[e-mail]