肾茶乙醇提取物抗氧化及对秀丽隐杆线虫的活性研究

Antioxidant activity of ethanol extract of kidney tea and its activity against Caenorhabditis elegans

摘要

肾茶（Clerodendranthus spicatus）作为传统中药，具有多种生物活性，近年来，肾茶体外抗氧化活性研究众多，但关于其乙醇提取物在生物体内的抗氧化活性及健康影响研究尚不充分。本研究通过测定肾茶乙醇提取物（KTe）对 DPPH、ABTS 自由基的清除能力来评价 KTe 体外抗氧化活性；以秀丽隐杆线虫（Caenorhabditis elegans）作为供试对象，测定 KTe 对秀丽隐杆线虫的寿命、产卵、孵化情况、脂褐素含量、运动能力等生理指标，以评估体内抗氧化活性。结果表明，KTe 具有良好的体外抗氧化活性，能促进低龄线虫各项生理指标，抑制老龄线虫各项生理指标；同时，可使得秀丽隐杆线虫的产卵量显著增加，但对孵化率无显著影响。所获得结果可为后续肾茶开发利用提供参考。

Abstract

Clerodendranthus spicatus (Kidney tea, KT), a traditional Chinese medicine, has a variety of biological activities. In recent years there are many studies on the antioxidant activity of kidney tea in vitro，but the research on the antioxidant activity and health effects of its ethanol extract in vivo is not sufficiently documented. In this case, the antioxidant activity of KTe (kidney tea ethanol extract) in vitro was evaluated by measuring the DPPH and ABTS free radical scavenging ability. Caenorhabditis elegans was used to evaluate the antioxidant activity of KTe in vivo. The physiological indexes, such as lifespan, oviposition and hatching, lipofuscin content and movement ability of C.elegans, were determined to evaluate the antioxidant tivity of KTe in vivo. The results showed that KTe had good antioxidant activity in vitro, which could promote the physiological indexes of young nematodes and inhibit the physiological indexes of old nematodes. At the same time, it can significantly increase the number of eggs laid by C.elegans, and has no significant effect on the hatching rate. All these results can provide reference for the subsequent development and utilization of KT.

关键词

肾茶 / 秀丽隐杆线虫 / 乙醇提取物 / 抗氧化 / 寿命

Key words

Clerodendranthus spicatus / Caenorhabditis elegans / ethanol extract / antioxidant / lifespan.

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基金资助

海南省自然科学基金(324RC454)