胶孢炭疽菌羧肽酶Y抑制蛋白CgPEB的功能初探

Functional analysis of carboxypeptidase Y inhibitor CgPEB of Colletotrichum gloeosporioides from Hevea brasiliensis

摘要

天然橡胶是中国重要的战略资源，由胶孢炭疽菌（Colletotrichum gloeosporioides）引起的炭疽病会严重影响橡胶产业的发展。开展橡胶树胶孢炭疽菌致病力相关基因的功能研究可以为病害防治提供理论基础。本研究在橡胶树胶孢炭疽菌中鉴定到一个编码羧肽酶Y抑制蛋白的基因Cgpeb。首先，通过原生质体转化体系构建了该基因的敲除突变株ΔCgpeb和互补菌株Res-ΔCgpeb，并对敲除突变株ΔCgpeb和互补菌株Res-ΔCgpeb营养生长以及致病能力相关表型进行了分析。研究发现Cgpeb基因敲除突变体ΔCgpeb菌落生长速率降低，分生孢子产量下降，且ΔCgpeb对橡胶树叶片和苹果的致病力明显下降。进一步分析发现，ΔCgpeb在洋葱表皮细胞中的入侵菌丝形成率显著降低。以上结果表明，Cgpeb在调控胶孢炭疽菌菌落生长、分生孢子产量以及致病能力中发挥重要作用。

Abstract

Natural rubber is an important strategic resource in China. The infection of Colletotrichum gloeosporides in rubber tree would greatly affect the development of rubber industry. The functional study of the pathogenicity-related genes of C. gloeosporioides can provide a theoretical basis for disease prevention and control. A gene CgPEB encoding carboxypeptidase Y inhibitor protein was identified in C. gloeosporioides. The gene knockout mutant strain was constructed according to the principle of homologous recombination, and the phenotype and pathogenicity analysis were performed. The results showed that the growth rate and conidial production of ΔCgpeb, Cgpeb gene knockout mutant were reduced. Besides, both the appressorium and invasive hyphae formation rate of ΔCgpeb were significantly reduced. These findings suggested that CgPEB plays an important role in regulating the colony growth, conidial production and pathogenicity of C. gloeosporioides.

关键词

胶孢炭疽菌 / CgPEB / 致病力 / 羧肽酶抑制子

Key words

Colletotrichum gloeosporioides / CgPEB / pathogenicity / carboxypeptidase Y inhibitor

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基金资助

国家自然科学基金项目(32260042)