铁皮石斛SWEET 基因家族鉴定及表达模式分析

Identification and expression pattern analysis of SWEET gene family from Dendrobium catenatum

摘要

糖转运蛋白 SWEET（sugars will eventually be exported transporter）在植物响应生物和非生物胁迫中起重要作用。为探讨铁皮石斛（Dendrobium catenatum）SWEET 基因的功能，基于铁皮石斛基因组数据，利用生物信息学方法对 SWEET 基因家族进行鉴定，并对其在干旱胁迫下的表达模式进行分析。结果显示，铁皮石斛中共有 27 个 DcSWEET 基因，理化性质分析表明，DcSWEET 蛋白质分子质量为 22.44～33.11 kDa，等电点为 8.47～9.63。系统发育分析表明，DcSWEET 蛋白可以划分为 4 个亚群，每个亚群具有保守的基序组成和基因结构。顺式作用元件分析表明，大量的生长发育相关元件、激素响应元件以及胁迫响应元件存在于DcSWEET 基因启动子序列上。RNA-seq 分析发现，17 个 DcSWEET 基因受干旱胁迫诱导上调表达。共表达网络分析显示，DcSWEET4、DcSWEET11、DcSWEET13、DcSWEET18、DcSWEET27 可能在铁皮石斛响应干旱胁迫中起主要作用。实时荧光定量 PCR 结果表明，茎和叶中的 DcSWEET11、DcSWEET13，以及叶中的DcSWEET18 基因受干旱胁迫显著上调表达，可作为铁皮石斛耐旱性的候选基因。本研究分析了铁皮石斛中响应干旱胁迫的关键 DcSWEET 基因，为将来提高铁皮石斛耐旱性提供分子依据。

Abstract

The sugar transporter SWEET (Sugars Will Eventually be Exported Transporter) plays a crucial role in plant responses to both biotic and abiotic stresses. To elucidate the function of the SWEET gene family in Dendrobium catenatum, 27 DcSWEET genes were identified and characterized from D. catenatum by using whole-genome data, and their expression patterns under drought stress were analyzed. The molecular weight of the DcSWEET proteins were found to range from 22.44 to 39.10 kDa, with heir isoelectric points being between 8.47 and 9.63. Phylogenetic analysis revealed that the DcSWEET proteins could be classified into four subgroups, each characterized by conserved motif compositions and gene structures. Cis-acting element analysis indicated that the promoters of DcSWEET genes contain various elements associated with hormone signaling, plant growth and development, light responsiveness, and stress response. RNA-seq analysis demonstrated that 17DcSWEET genes were up-regulated under drought stress. Coexpression network analysis suggested that DcSWEET4, DcSWEET11, DcSWEET13, DcSWEET18, and DcSWEET27 may play pivotal roles in response to drought stress in D. catenatum. RT-qPCR confirmed that DcSWEET11, DcSWEET13, and DcSWEET18 were significantly up-regulated in stems and leaves under drought conditions, making them potential candidate genes for enhancing drought tolerance in D. catenatum. This study provides a comprehensive analysis of key SWEET genes involved in drought stress responses in D. catenatum, offering a molecular foundation for future efforts to improve its drought resistance.

关键词

铁皮石斛 / SWEET 基因家族 / 干旱胁迫 / 表达分析

Key words

Dendrobium catenatum / SWEET gene family / drought stress / expression pattern

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基金资助

海南省自然科学基金项目(319MS009)

海南大学横向课题(HD-KYH-2023093)

海南省普通高等学校研究生创新科研课题(Qhys2023-259)