海南桃蚜对拟除虫菊酯类药剂抗性监测及电压门控钠离子通道突变分析

Resistance monitoring and voltage-gated sodium channel mutation analysis of Myzus persicae against pyrethroids in Hainan

摘要

桃蚜（Myzus persicae）是一种全球分布的多食性害虫，可对多种经济作物造成严重危害。为评估海南桃蚜田间种群对拟除虫菊酯类药剂的抗性水平，本研究对 2021-2023 年海口、儋州和三亚地区的桃蚜田间种群进行了抗药性监测。叶片药膜法测定桃蚜对高效氯氟氰菊酯、溴氰菊酯、联苯菊酯和氯菊酯敏感性的结果表明，3 个地区的桃蚜种群均对高效氯氟氰菊酯的抗性水平最低，对联苯菊酯的抗性最高，且整体抗药性水平随时间呈上升趋势。其中，2021 年三亚种群对高效氯氟氰菊酯的抗性最低，LC₅₀ 值为 41.992 mg·L⁻¹；海口种群对联苯菊酯的抗性增长最为显著，抗性倍数从 2021 年的 7.35 倍提升至 2023 年的 85.32 倍，其 LC₅₀ 值在 2023 年达到 2 085.829 mg·L⁻¹。检测桃蚜钠离子通道 Domain Ⅱ区域和辅助亚基 TipE 的突变情况发现，2022 年海口、儋州和三亚种群中 TipE 基因未发生突变，但两个经典突变位点 918 和 1 014 出现三种突变连锁现象（I-F、T-F、L-L）。其中，L-L 出现频率最高，分别达到 70%、50% 和 50%，并且三种连锁突变形式与不同菊酯药剂的抗性水平呈现一致的变化趋势。本研究揭示了海南地区桃蚜田间种群的抗药性现状及抗性基因突变情况，为科学制定抗性管理策略提供重要参考，同时为深入探究桃蚜对拟除虫菊酯类药剂抗性形成机制提供了素材。

Abstract

Myzus persicae is a globally distributed polyphagous pest that can cause severe damage to a variety of economic crops. To assess the resistance levels of field populations of M. persicae to pyrethroid insecticides in Hainan Province, resistance of M. persicae to pyrethroid insecticides was monitored in Haikou, Danzhou, and Sanya from 2021 to 2023. The leaf-dip method was used to determine the sensitivity of M. persicae to lambda-cyhalothrin, deltamethrin, bifenthrin, and permethrin. The results showed that the field populations in all the three regions exhibited the lowest resistance to lambda-cyhalothrin and the highest resistance to bifenthrin, with an overall increasing trend in resistance levels over time. In 2021, the Sanya population showed the lowest resistance to lambda-cyhalothrin, with the LC₅₀ value being 41.992 mg·L⁻¹. The Haikou population exhibited the most significant increase in resistance to bifenthrin, with the resistance ratio rising from 7.35-fold in 2021 to 85.32-fold in 2023, and the LC₅₀ value reaching 2 085.829 mg·L⁻¹ in 2023. Analysis of mutations in the sodium ion channel Domain Ⅱ region and the auxiliary subunit TipE revealed that no mutations occurred in the TipE gene in the Haikou, Danzhou, and Sanya populations in 2022. However, mutation of three interlock patterns (I-F, T-F, L-L) was observed at the classical mutation sites 918 and 1014. Among these, the L-L pattern was the most frequent, reaching 70%, 50%, and 50% in the respective populations. The mutation of three interclock patterns showed consistent trends with the resistance levels to different pyrethroid insecticides. These findings reveal the current resistance status and mutation profiles ofM. persicae field populations in Hainan, providing important references for the scientific formulation of resistance management strategies and materials for further research into the mechanisms of pyrethroid resistance in M. persicae.

关键词

桃蚜 / 拟除虫菊酯 / 抗药性监测 / 突变点检测

Key words

Myzus persicae / pyrethroid / resistance monitoring / mutation detection

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基金资助

菁英人才科技项目(SCKJ-JYRC-2023-15)

国家自然科学基金项目(32260666)

海南省冬季瓜菜产业技术体系项目(HNARS-05-G04)