康欣口服液对衰老大鼠氧化应激和肠道菌群的影响 ^*

Effect of Kangxin Oral Liquid on aging rat antioxidant and intervention imbalance of gut microbiota

摘要

目的 通过研究康欣口服液对衰老大鼠氧化应激及肠道菌群的影响, 初步探讨其抗衰老机制。 方法 建立 D-半乳糖诱导的衰老大鼠模型, 灌胃给予康欣口服液, 监测体质量及测定脑、 脾、 肝和肾脏器指数; 测定血清和各脏器中氧化应激指标超氧化物歧化酶 (SOD)、 丙二醛 (MDA) 和活性氧 (ROS) 水平; 取结肠内容物进行 16S rRNA 测序, 研究康欣口服液对衰老所致肠道菌群改变的影响; 分析肠道菌群与氧化应激指标间的相关性。 结果 康欣口服液能够明显增加衰老大鼠的体质量及各脏器指数; 减少血清和各脏器中氧化应激因子水平; 升高 Pseudoflavonifractor, Ruminococcaceae 等有益菌丰度, 降低 Clostridiales 等有害菌丰度, 维持衰老相关的肠道菌群稳态; Clostridiales、 Pseudoflavonifractor 和 Ruminococcaceae 丰度与 SOD、MDA 和 ROS 水平呈显著相关。 结论 康欣口服液可能通过减轻氧化应激损伤, 调节肠道菌群改变, 进而发挥抗衰老作用。

Abstract

Objective This study preliminarily discussed the anti-aging mechanism of Kangxin Oral Liquid by studying its effects on oxidative stress and intestinal flora in aging rats. Methods The aging rat model was established by D-galactose induction, and Kangxin Oral Liquid was given by gavage at the same time. The body weight was monitored and the indexes of the brain, spleen, liver and kidney were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and eactive oxygen species (ROS) in serum and organs were measured. The effect of Kangxin oral liquid on the changes in gut microbiota caused by aging was studied by 16S rRNA sequencing. The correlation between intestinal flora and oxidative stress indexes was analyzed. Results Kangxin oral liquid could significantly increase the body weight and organ index of aging rats, reduce the levels of oxidative stress factors in serum and organs, increase the abundance of beneficial bacterias such as Pseudoflavonifractor and Ruminococcaceae, reduce the abundance of harmful bacterias such as Clostridiales, and maintain the homeostasis of aging-related gut microbiota; the abundance of Clostridiales, Pseudoflavonifractor and Ruminococcaceae was significantly correlated with SOD, MDA and ROS levels. Conclusion Kangxin Oral Liquid can anti-aging by reducing oxidative stress injury and regulating intestinal microbial changes.

关键词

康欣口服液 / 衰老 / D-半乳糖 / 氧化应激 / 肠道菌群

Key words

Kangxin Oral Liquid / Aging / D-galactose / Oxidative stress / Gut microbiota

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基金资助

^*吉林省科技发展计划项目(20220401061YY)