In vitro expression of human interleukin 10 in periodontal ligament of rats—using hydrodynamic tail vein injection

In vitro expression of human interleukin 10 in periodontal ligament of rats—using hydrodynamic tail vein injection

摘要

ObjectiveTo investigate the expression of Human Interleukin 10（IL 10） gene in the periodontal ligament of rats via hydrodynamic tail vein injection.MethodsTwenty four Sprague Dawley（SD） rats were randomly divided into hIL 10 group and the vector group.After plasmid was introduced into rats via hydrodynamic tail vein injection, frozen liver sections and serum were used to detect the exogenous Human Interleukin 10 gene expression.The periodontal ligament at furcation area of maxillary second molar was taken for examination of hIL 10 expression by immunohistochemistry.Result Frozen liver sections showed a large number of bright green fluorescent proteins, and hIL 10 proteins were highly expressed in the liver 24h after injection.The expression of hIL 10 protein could be detected in serum and peaked at 8h after injection and no obvious hIL 10 expression was found in periodontal ligament in hIL 10 group.No expression of hIL 10 was detected in vector group.Conclusion A relatively stable expression of exogenous gene in liver can be obtained via hydrodynamic tail vein injection in rats；however, expression of hIL 10 protein was not found in the periodontal ligament.